FastQCFastQC Report
Sun 21 May 2023
EGAF00007848191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49856
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20964.2041078305519894No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15953.199213735558408No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15613.1310173299101414No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11352.27655648267009No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8211.6467426187419767No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7671.5384306803594352No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7541.5123555840821565No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7201.4441591784338896No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6891.3819801026957639No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6811.3659338896020539No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6101.2235237483953787No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5821.167362002567394No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5751.153321566110398No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5551.1132060333761233No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5531.1091944801026958No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5281.0590500641848524No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5281.0590500641848524No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5211.0450096277278562No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5151.0329749679075737No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4970.9968709884467266No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4880.978818998716303No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4420.8865532734274711No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4390.8805359435173299No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4340.8705070603337614No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4330.8685012836970475No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4290.8604781771501926No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4240.8504492939666239No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4230.8484435173299101No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4100.8223684210526315No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4010.8043164313222081No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3850.7722240051347882No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3840.7702182284980745No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3830.7682124518613607No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3810.7642008985879333No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3780.758183568677792No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3600.7220795892169448No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3590.7200738125802311No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3580.7180680359435173No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3550.7120507060333762No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3460.6939987163029525No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3220.6458600770218229No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3200.6418485237483954No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3170.6358311938382541No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2930.5876925545571245No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2900.5816752246469833No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2880.5776636713735559No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2880.5776636713735559No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2560.5134788189987163No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2500.5014441591784339No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2420.48539794608472403No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2410.4833921694480102No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2380.477374839537869No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2340.4693517329910141No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2210.4432766367137356No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2210.4432766367137356No Hit
TGTGCCTGTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2190.4392650834403081No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2100.42121309370988447No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2050.41118421052631576No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1990.3991495507060334No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1950.3911264441591784No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1870.37508023106546856No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.37508023106546856No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.3730744544287548No Hit
TGTGCCTGTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1840.36906290115532736No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1720.3449935815147625No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1700.34098202824133506No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1690.3389762516046213No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1680.33697047496790755No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1660.3329589216944801No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1640.3289473684210526No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1630.3269415917843389No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1530.30688382541720155No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.2988607188703466No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1470.29484916559691915No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1470.29484916559691915No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1450.29083761232349165No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1440.28883183568677795No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1410.2828145057766367No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1390.27880295250320924No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.27479139922978174No Hit
TGTGCCTGTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1360.27278562259306804No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1310.26275673940949934No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1280.25673940949935814No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1280.25673940949935814No Hit
TGTGCCTGTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.2547336328626444No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1260.2527278562259307No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1260.2527278562259307No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1240.24871630295250324No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.24671052631578946No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1230.24671052631578946No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1230.24671052631578946No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1230.24671052631578946No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1210.24269897304236202No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1200.2406931964056483No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1150.23066431322207961No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1140.22865853658536583No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1130.2266527599486521No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.22264120667522466No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1090.21862965340179716No Hit
TGTGCCTGTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1080.21662387676508343No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1080.21662387676508343No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1080.21662387676508343No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1030.20659499358151476No Hit
TGTGCCTGTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.20458921694480103No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.20057766367137356No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1000.20057766367137356No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC980.19656611039794608No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC970.19456033376123236No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA930.1865372272143774No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA920.18453145057766368No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA920.18453145057766368No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.18252567394094993No Hit
TGTGCCTGTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.174502567394095No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT810.16246790757381258No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA810.16246790757381258No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT790.1584563543003851No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC790.1584563543003851No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC770.15444480102695762No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC750.15043324775353015No Hit
TGTGCCTGTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.15043324775353015No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC730.1464216944801027No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT730.1464216944801027No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.14441591784338897No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT710.14241014120667522No Hit
TGTGCCTGTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.1404043645699615No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC690.13839858793324777No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA690.13839858793324777No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT680.13639281129653402No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.13238125802310655No Hit
TGTGCCTGTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT660.13238125802310655No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA660.13238125802310655No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA660.13238125802310655No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.13238125802310655No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.13037548138639282No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT630.12636392811296535No Hit
TGTGCCTGTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC630.12636392811296535No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.12435815147625162No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA610.12235237483953787No Hit
TGTGCCTGTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.12235237483953787No Hit
TGTGCCTGTCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.12034659820282415No Hit
TGTGCCTGTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT600.12034659820282415No Hit
TGTGCCTGTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.12034659820282415No Hit
TGTGCCTGTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC590.1183408215661104No Hit
TGTGCCTGTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT590.1183408215661104No Hit
TGTGCCTGTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC590.1183408215661104No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.1183408215661104No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA580.11633504492939667No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG580.11633504492939667No Hit
TGTGCCTGTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC570.11432926829268292No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.1123234916559692No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA560.1123234916559692No Hit
TGTGCCTGTCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT550.11031771501925544No Hit
TGTGCCTGTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC540.10831193838254172No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC540.10831193838254172No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA530.10630616174582799No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG520.10430038510911424No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA520.10430038510911424No Hit
TGTGCCTGTCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT510.10229460847240052No Hit
TGTGCCTGTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.10229460847240052No Hit
TGTGCCTGTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT500.10028883183568678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)