FastQCFastQC Report
Sun 21 May 2023
EGAF00007848198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112784
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA52674.669988650872464No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA37903.3604057313094056No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA33953.010178748758689No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA29092.5792665626329976No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG28692.543800539083558No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA26282.330117747198184No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA22311.9781174634699958No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG20981.860192935168109No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT19771.7529082139310541No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT19731.7493616115761101No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG19671.744041708043694No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG19581.7360618527450702No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19411.7209887927365584No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG16441.457653567881969No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA14721.3051496666193787No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14181.257270534827635No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT14141.2537239324726912No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT13691.2138246559795716No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13171.1677188253653001No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12851.1393460065257484No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT12481.1065399347425167No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG12471.1056532841537807No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12441.1029933323875727No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG12381.0976734288551568No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT11531.0223081288125975No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11511.0205348276351256No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG11270.9992552135054616No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT11080.9824088523194779No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10680.9469428287700383No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG10580.9380763228826784No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9790.8680309263725351No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT9180.8139452404596397No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9090.8059653851610158No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9010.7988721804511277No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT8900.789119023975032No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8080.7164136756986806No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG7670.6800610015605051No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7580.6720811462618812No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT7430.6587813874308412No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT7300.6472549297772734No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7220.6401617250673854No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT6960.6171088097602497No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6790.6020357497517379No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT6670.5913959426869059No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT6620.586962689743226No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6260.5550432685487303No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA6180.5479500638388424No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT5480.48588452262732296No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT5140.45573840261029935No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5080.45041849907788334No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4990.44243864377925946No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT4690.41583912611717977No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4540.40253936728613987No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4440.39367286139877994No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4430.392786210810044No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4400.390126259043836No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT4400.390126259043836No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4380.38835295786636403No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT4140.3670733437367002No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA4090.3626400907930203No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4080.3617534402042843No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3870.3431337778408285No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3850.3413604766633565No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3820.3387005248971485No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3800.3369272237196766No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3780.33515392254220455No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3620.3209675131224287No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3550.31476095900127676No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3370.29880124840402894No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT3300.292594694282877No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3160.28018158604057314No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3150.27929493545183715No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3110.2757483330968932No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3090.2739750319194212No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3050.27042842956447727No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT3050.27042842956447727No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2990.26510852603206125No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2870.2544687189672294No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2830.2509221166122855No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2740.24294226131366148No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2730.24205561072492554No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2730.24205561072492554No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2690.23850900836998157No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2670.23673570719250958No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2660.2358490566037736No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2630.23318910483756558No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2620.23230245424882964No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2620.23230245424882964No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2400.21279614129663785No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2350.20836288835295785No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2330.20658958717548587No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2310.20481628599801388No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2280.20215633423180593No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2280.20215633423180593No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2240.19860973187686196No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2240.19860973187686196No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2230.19772308128812596No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2220.19683643069938997No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2190.19417647893318202No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2170.19240317775571003No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2070.1835366718683501No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2030.17999006951340615No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT2020.17910341892467016No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.1782167683359342No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.1782167683359342No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG2000.1773301177471982No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1990.1764434671584622No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1920.17023691303731026No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1910.16935026244857426No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1890.16757696127110228No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1850.16403035891615833No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1830.16225705773868634No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1760.1560505036175344No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1760.1560505036175344No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1750.1551638530287984No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1740.15427720244006243No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1740.15427720244006243No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1690.14984394949638247No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1690.14984394949638247No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1690.14984394949638247No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1660.1471839977301745No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1640.1454106965527025No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1620.14363739537523054No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1590.14097744360902253No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1570.13920414243155058No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1570.13920414243155058No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1560.13831749184281458No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT1520.13477088948787064No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1470.13033763654419067No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1460.12945098595545468No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1460.12945098595545468No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.1276776847779827No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1420.12590438360051073No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1390.12324443183430274No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT1380.12235778124556675No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1380.12235778124556675No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG1360.12058448006809477No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1360.12058448006809477No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1320.11703787771315079No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1290.11437792594694283No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1250.11083132359199888No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1200.10639807064831892No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1200.10639807064831892No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1200.10639807064831892No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1190.10551142005958292No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1190.10551142005958292No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.10462476947084692No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1140.10107816711590296No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1140.10107816711590296No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1140.10107816711590296No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.10019151652716697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)