FastQCFastQC Report
Sun 21 May 2023
EGAF00007848199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112784
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA50394.467832316640658No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA34793.0846573982125123No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA31252.7707830897999717No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA26282.330117747198184No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT24862.2042133635976735No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG24772.1962335082990494No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT21721.9258050787345722No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA21271.8859058022414525No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19331.7138955880266704No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG18881.6739963115335508No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG18831.6695630585898709No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG17511.5525251808767202No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT17441.5463186267555682No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA17141.5197191090934885No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT15811.4017945807916017No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14861.3175627748616825No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG14091.249290679529011No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT13871.2297843665768193No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13261.175698680663924No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13121.16328557242162No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG13021.1544190665342602No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT12491.1074265853312526No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT12051.0684139594268691No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA12001.0639807064831892No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT11831.0489076464746772No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11571.0258547311675414No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11501.0196481770463897No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG10760.9540360334799263No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT10310.9141367569868066No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10310.9141367569868066No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG9940.8813306852035749No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT9760.8653709746063271No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9090.8059653851610158No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8230.7297134345297205No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8100.7181869768761526No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8070.7155270251099447No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT8040.7128670733437368No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT7550.6694211944956732No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT7400.6561214356646333No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT7340.6508015321322174No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7300.6472549297772734No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT7240.6419350262448574No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG7110.6304085685912895No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7010.6215420627039296No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT7010.6215420627039296No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT6860.6082423038728898No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT6120.5426301603064264No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT5730.5080507873457228No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5550.49209107674847496No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5550.49209107674847496No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT5060.44864519790041135No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5000.4433252943679955No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4970.44066534260178747No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT4830.4282522343594836No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4710.41761242729465176No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4610.4087459214072918No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4550.40342601787487586No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT4370.38746630727762804No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4360.38657965668889205No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4240.37593984962406013No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT4210.3732798978578522No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT4070.3608667896155483No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT3870.3431337778408285No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3840.3404738260746205No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3730.3307206695985246No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3710.3289473684210526No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3670.3254007660661087No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT3670.3254007660661087No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3620.3209675131224287No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA3560.31564760959001276No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3540.31387430841254077No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3440.3050078025251809No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT3420.3032345013477089No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3370.29880124840402894No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3350.29702794722655695No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT3300.292594694282877No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3230.2863881401617251No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3230.2863881401617251No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2930.25978862249964535No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2930.25978862249964535No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2920.25890197191090936No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2830.2509221166122855No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2760.2447155624911335No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2760.2447155624911335No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2740.24294226131366148No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2650.2349624060150376No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2610.23141580366009365No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2580.22875585189388567No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2560.22698255071641366No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2550.22609590012767766No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2500.22166264718399775No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2480.21988934600652577No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2440.21634274365158176No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2440.21634274365158176No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2290.20304298482054195No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2260.20038303305433394No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2230.19772308128812596No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2220.19683643069938997No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2200.195063129521918No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2200.195063129521918No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT2190.19417647893318202No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2190.19417647893318202No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2160.19151652716697404No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2130.18885657540076606No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2100.1861966236345581No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2090.1853099730458221No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2090.1853099730458221No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1990.1764434671584622No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1970.1746701659809902No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1940.17201021421478224No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1940.17201021421478224No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1930.17112356362604625No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1830.16225705773868634No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1830.16225705773868634No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1740.15427720244006243No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1700.15073060008511846No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG1690.14984394949638247No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.14807064831891048No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1670.14807064831891048No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1620.14363739537523054No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1580.14009079302028657No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1540.1365441906653426No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1500.13299758831039865No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1490.13211093772166263No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1480.13122428713292664No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1470.13033763654419067No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1470.13033763654419067No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1450.12856433536671869No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT1430.1267910341892467No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1420.12590438360051073No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1400.12413108242303873No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1390.12324443183430274No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1390.12324443183430274No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1360.12058448006809477No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1350.11969782947935878No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.11881117889062279No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1340.11881117889062279No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1330.1179245283018868No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1320.11703787771315079No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1320.11703787771315079No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1320.11703787771315079No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1290.11437792594694283No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG1290.11437792594694283No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1260.11171797418073487No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1250.11083132359199888No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1250.11083132359199888No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1230.10905802241452688No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1210.10728472123705489No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1190.10551142005958292No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT1190.10551142005958292No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1160.10285146829337494No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1150.10196481770463897No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1130.10019151652716697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)