FastQCFastQC Report
Sun 21 May 2023
EGAF00007848221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58809
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA32395.507660392116852No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22053.7494261082487372No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA17653.001241306602731No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT14552.4741111054430447No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11301.9214746042272441No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG11071.882364944141203No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10141.7242258837932969No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9901.6834158037035147No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8931.5184750633406452No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8831.5014708633032359No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8771.4912683432807903No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT8201.3943444030675578No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7961.3535343229777757No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7651.300821302861807No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7561.2855175228281386No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7541.2821166828206567No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7111.208998622659797No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT6721.142682242513901No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6481.1018721624241188No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6331.076365862368005No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6141.0440578822969273No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5790.984543182165995No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5650.9607373021136221No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5570.9471339420836947No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5230.8893196619565032No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT5030.8553112618816848No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5020.8536108418779439No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4890.8315053818293119No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4720.8025982417657161No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4710.8008978217619752No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4650.7906953017395296No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4640.7889948817357887No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4620.7855940417283069No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4620.7855940417283069No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4460.758387321668452No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4320.7345814416160792No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4030.6852692615075924No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3830.651260861432774No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3800.6461596014215512No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3780.6427587614140693No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3670.6240541413729191No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3340.5679402812494686No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3310.5628390212382458No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3260.5543369212195413No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3200.5441344011970957No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3190.5424339811933547No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.5118264211260182No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2970.5050247411110544No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2890.49142138108112704No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2810.47781802105119964No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2720.46251424101753136No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2640.4489108809876039No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2540.4319066809501947No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2530.43020626094645376No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2460.41830332092026734No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.4081008008978218No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2370.402999540886599No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2310.39279702086415347No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.3825945008417079No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2190.37239198081926234No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2100.35708820078559406No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.3417844207519257No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1990.3383835807444439No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1970.33498274073696205No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1930.3281810607219984No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1890.32137938070703465No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1790.3043751806696254No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1760.29927392065840264No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1720.29247224064343896No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1710.290771820639698No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1700.2890714006359571No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.28567056062847523No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1660.2822697206209934No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1650.28056930061725244No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1630.27716846060977063No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1600.27206720059854783No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1580.26866636059106597No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1540.2618646805761023No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1520.25846384056862043No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.25506300056113856No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1500.25506300056113856No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1500.25506300056113856No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1480.25166216055365676No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1480.25166216055365676No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1470.24996174054991582No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1420.24145964053121122No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.23295754051250658No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.22105460048632014No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1280.21765376047883828No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1270.21595334047509734No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1210.2057508204526518No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1210.2057508204526518No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.2057508204526518No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.2040504004489109No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1190.20234998044516994No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT1190.20234998044516994No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1180.20064956044142904No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1170.19894914043768813No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1150.19554830043020627No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1140.19384788042646534No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1140.19384788042646534No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.1904470404189835No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1110.18874662041524257No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1110.18874662041524257No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1080.1836453604040198No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1080.1836453604040198No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.18194494040027887No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1010.17174242037783333No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1000.1700420003740924No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.16494074036286963No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA950.1615399003553878No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.1615399003553878No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC950.1615399003553878No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC950.1615399003553878No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA940.15983948035164686No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA930.15813906034790592No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT910.1547382203404241No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT880.14963696032920132No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA860.14623612032171948No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA820.13943444030675578No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT800.13603360029927392No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA800.13603360029927392No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA790.13433318029553298No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.13263276029179205No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC780.13263276029179205No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC770.13093234028805115No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT770.13093234028805115No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.12923192028431021No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT760.12923192028431021No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.12753150028056928No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA740.12583108027682838No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA740.12583108027682838No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG740.12583108027682838No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA720.12243024026934651No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC700.11902940026186468No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT700.11902940026186468No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC700.11902940026186468No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA690.11732898025812374No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.11732898025812374No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.11732898025812374No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA680.11562856025438284No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC680.11562856025438284No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.11392814025064191No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT670.11392814025064191No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT640.10882688023941914No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.10882688023941914No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA630.10712646023567822No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC630.10712646023567822No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA630.10712646023567822No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA620.10542604023193729No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC620.10542604023193729No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA610.10372562022819636No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.10032478022071452No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.10032478022071452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)