FastQCFastQC Report
Sun 21 May 2023
EGAF00007848225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70763
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA32734.6252985317185535No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA31944.51365826773879No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT17542.478696493930444No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17522.4758701581334877No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16482.3289006966917736No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13381.8908186481635885No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA13341.8851659765696762No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12831.8130944137472973No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11871.6774302954934075No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10161.4357785848536664No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10101.4272995774627983No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT10011.4145810663764962No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT9231.3043539702952107No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9231.3043539702952107No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT8801.243587750660656No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG8281.170103019939799No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8221.161624012548931No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG8161.153145005158063No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7831.1065104645082882No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7601.0740076028432939No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7591.0725944349448158No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7281.0287862300919972No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7261.0259598942950412No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT6910.9764990178483106No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6760.9553014993711404No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6740.9524751635741843No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6540.9242118056046239No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6510.9199723019091898No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5810.8210505490157286No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5760.8139847095233385No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5740.8111583737263826No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5590.7899608552492122No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5400.7631106651781299No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT5350.7560448256857397No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5200.7348473072085695No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5200.7348473072085695No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT5100.7207156282237893No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5030.7108234529344432No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4980.703757613442053No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4760.6726679196755366No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4640.6557099048938004No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4440.6274465469242401No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4150.5864646778683775No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3950.5582013198988172No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3880.548309144609471No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3870.546895976710993No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3860.5454828088125151No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3640.5143931150459986No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3400.48047708548252616No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3390.47906391758404815No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3360.47482441388861407No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3350.4734112459901361No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3290.46493223859926797No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3150.4451478880205757No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3140.4437347201220977No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3130.44232155222361974No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3020.42677670534036155No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3000.4239503695434054No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2980.42112403374644947No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2670.37731582889363086No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2510.3547051425179826No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2440.34481296722863647No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2410.3405734635332024No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2380.3363339598377683No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2360.3335076240408123No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2110.29817842657886184No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.29393892288342777No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2050.28969941918799375No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.2868730833910377No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.2868730833910377No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1990.2812204117971256No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1970.2783940760001696No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1960.27698090810169157No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1950.2755677402032135No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1920.2713282365077795No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1890.26708873281234546No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1860.2628492291169114No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1840.2600228933199553No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1830.2586097254214773No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1760.2487175501321312No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1740.24589121433517516No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1730.24447804643669716No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1710.2416517106397411No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1700.24023854274126308No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.24023854274126308No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.22752003165496093No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1610.22752003165496093No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1570.22186736006104885No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1530.21621468846713676No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1510.21338835267018078No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1460.20632251317779066No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1460.20632251317779066No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1440.20349617738083461No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.20349617738083461No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.19360400209148848No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1370.19360400209148848No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1350.19077766629453247No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.19077766629453247No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1340.18936449839605443No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.1794723231067083No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1270.1794723231067083No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1260.1780591552082303No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.17381965151279624No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1220.17240648361431823No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1210.1709933157158402No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1210.1709933157158402No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.16675381202040615No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1160.1639274762234501No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1140.16110114042649407No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1130.15968797252801606No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.15968797252801606No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1130.15968797252801606No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.15686163673106002No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1110.15686163673106002No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1100.155448468832582No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1100.155448468832582No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.155448468832582No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.15262213303562597No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1080.15262213303562597No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1070.15120896513714793No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.15120896513714793No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1040.14696946144171388No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1040.14696946144171388No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1010.14272995774627983No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.14272995774627983No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1000.14131678984780183No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1000.14131678984780183No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA990.1399036219493238No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA970.13707728615236775No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.13566411825388974No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA930.1314246145584557No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC910.12859827876149965No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT910.12859827876149965No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC880.1243587750660656No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT870.12294560716758758No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.12294560716758758No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT850.12011927137063154No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT850.12011927137063154No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC840.11870610347215352No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA820.11587976767519748No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA810.11446659977671948No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT800.11305343187824146No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.11164026397976344No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.11022709608128542No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA770.1088139281828074No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.1088139281828074No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT760.10740076028432938No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA730.10316125658889533No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.10033492079193929No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.10033492079193929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)