FastQCFastQC Report
Sun 21 May 2023
EGAF00007848237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62376
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG22323.5782993459022703No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA20963.360266769270232No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT20153.2304091317173276No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG20043.2127741439015005No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA19073.0572656149801207No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14882.38553289726818No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12582.016801333846351No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11571.8548800820828524No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10911.7490701551878929No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10631.7041810952930614No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10061.6127997947928692No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9741.561498012055919No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9561.5326407592663844No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8491.3611004232397075No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7841.2568936770552777No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6821.093369244581249No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6741.0805437988970117No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6641.0645119917917147No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT6261.0035911247915865No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6160.9875593176862896No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6060.9715275105809927No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6030.9667179684494036No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5900.9458766192125175No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5830.9346543542388098No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA5520.8849557522123894No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5400.865717583686033No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA5300.8496857765807362No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT5030.8063998973964346No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4980.798383993843786No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4940.7919712710016673No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4640.7438758496857766No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4250.6813518019751186No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4120.6605104527382327No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3920.6284468385276388No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3780.6060023085802232No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3540.5675259715275106No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3510.5627164293959215No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3340.5354623573169168No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3310.5306528151853277No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3130.5017955623957933No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3070.49217647813261506No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3030.48576375529049637No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2990.47935103244837757No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.47774785173784784No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2970.4761446710273182No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2950.47293830960625877No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2950.47293830960625877No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2910.4665255867641401No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2790.4472874182377838No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2760.4424778761061947No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2760.4424778761061947No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2750.440874695395665No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2660.4264460690008978No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2550.40881108118507115No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2430.3895729126587149No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2360.37835064768500704No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2300.3687315634218289No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC2270.3639220212902398No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2260.36231884057971014No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2200.352699756316532No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2180.3494933948954726No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.3494933948954726No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2170.3478902141849429No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2150.3446838527638836No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2070.33185840707964603No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2040.32704886494805696No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2000.32063614210593816No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1950.3126202385532897No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.30460433500064127No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1860.2981916121585225No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1770.2837629857637553No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1760.28215980505322563No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1740.27895344363216623No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1700.2725407207900475No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1690.27093754007951776No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.2677311786584584No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1580.25330255226369114No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1570.25169937155316147No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.2500961908426318No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1550.24849301013210207No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1520.243683468000513No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1510.24208028728998332No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1510.24208028728998332No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1510.24208028728998332No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1500.24047710657945365No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.23727074515839425No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1480.23727074515839425No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1470.23566756444786457No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1450.23246120302680517No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1420.2276516608952161No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1390.22284211876362703No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1380.22123893805309736No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1360.21803257663203796No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1300.20841349236885984No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1210.1939848659740926No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1210.1939848659740926No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1200.19238168526356292No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1180.18917532384250352No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1170.18757214313197382No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.18596896242144415No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.18596896242144415No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1140.18276260100038477No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1120.17955623957932537No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1100.176349878158266No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.176349878158266No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1100.176349878158266No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.176349878158266No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.1747466974477363No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1080.17314351673720663No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1070.17154033602667693No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1060.16993715531614725No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.16833397460561755No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1050.16833397460561755No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1000.16031807105296908No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC980.1571117096319097No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC970.15550852892138No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA970.15550852892138No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC950.15230216750032063No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT940.15069898678979093No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.14749262536873156No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC920.14749262536873156No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA910.14588944465820186No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT910.14588944465820186No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA880.14107990252661282No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT860.13787354110555342No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA850.13627036039502374No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA850.13627036039502374No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA840.13466717968449404No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.13306399897396434No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA820.13146081826343464No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT820.13146081826343464No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.12985763755290494No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC800.12825445684237527No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.12825445684237527No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC790.12665127613184557No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT790.12665127613184557No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC780.1250480954213159No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC760.1218417340002565No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT740.11863537257919712No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.11703219186866745No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC710.11382583044760805No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC710.11382583044760805No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA690.11061946902654868No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT680.10901628831601898No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC680.10901628831601898No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA650.10420674618442992No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.10420674618442992No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.10420674618442992No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA640.10260356547390022No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.10260356547390022No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA640.10260356547390022No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.10100038476337052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)