FastQCFastQC Report
Sun 21 May 2023
EGAF00007848251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72351
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT15812.185180578015508No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT15462.1368052964022612No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA13921.9239540573039764No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA13481.8631394175616094No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13291.8368785504001326No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG12751.7622424016254092No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12641.7470387416898177No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT12151.6793133474312727No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12041.664109687495681No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11791.6295559149147905No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA11561.5977664441403712No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11251.554919766140067No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10841.498251579107407No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA10631.469226410139459No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9251.2784895854929441No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9141.2632859255573523No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8591.1872676258793935No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8091.1181600807176129No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7951.098809968072314No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7711.0656383463946593No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA7601.0504346864590677No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7491.0352310265234759No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7361.017263064781413No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7351.0158809138781772No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7261.0034415557490568No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7241.0006772539425854No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7210.9965308012328786No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7080.9785628394908156No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6910.9550662741358101No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6830.9440090669099251No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6810.941244765103454No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6520.9011623889096212No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6400.8845765780707938No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6070.8389655982640184No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5990.8279083910381336No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5400.7463614877472322No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA5340.7380685823278186No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5270.7283935260051692No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5090.7035148097469281No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA4980.6883111498113363No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4900.6772539425854515No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4630.6399358681980899No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4540.6274965100689693No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT4530.6261143591657337No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4530.6261143591657337No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4500.621967906456027No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4460.6164393028430845No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4350.6012356429074927No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4250.5874141338751365No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4050.5597711158104242No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3940.5445674558748324No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3800.5252173432295338No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA3780.5224530414230625No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3690.510013683293942No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3580.4948100233583503No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3560.492045721551879No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3460.4782242125195229No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3420.4726956089065804No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3320.45887409987422423No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3250.449199043551575No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3230.4464347417451038No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3220.4450525908418681No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3200.4422882890353969No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3180.4395239872289257No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3100.4284667800030407No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2870.3966773092286216No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.3939130074221504No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2720.37594504568008735No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2630.3635056875509668No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2630.3635056875509668No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2560.35383063122831754No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2380.3289519149700764No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2360.3261876131636052No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2340.323423311357134No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2300.3178947077441915No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2280.3151304059377203No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2240.3096018023247778No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2220.30683750051830655No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2200.30407319871183536No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2120.2930159914859504No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2120.2930159914859504No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2040.28195878426006554No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.2791944824535943No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.27366587884065185No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1930.26675512432447374No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1920.26537297342123817No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1850.2556979170985888No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1830.25293361529211755No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1820.251551464388882No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1820.251551464388882No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1730.23911210625976145No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1730.23911210625976145No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1650.22805489903387652No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1650.22805489903387652No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1610.22252629542093405No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1520.21008693729181352No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1520.21008693729181352No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.20870478638857792No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1490.20594048458210668No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1430.197647579162693No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1420.19626542825945734No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1330.18382607013033683No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1270.17553316471092315No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1260.17415101380768752No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1220.16862241019474505No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1190.1644759574850382No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1170.161711655678567No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.1548009011623889No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1120.1548009011623889No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1110.15341875025915327No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.15065444845268205No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.1465079957429752No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1010.13959724122679715No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG1010.13959724122679715No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA990.13683293942032593No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.1354507885170903No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC980.1354507885170903No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT970.13406863761385468No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA960.13268648671061908No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG960.13268648671061908No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC930.1285400340009122No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT920.12715788309767662No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG890.12301143038796974No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA870.12024712858149852No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.1188649776782629No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT860.1188649776782629No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT850.1174828267750273No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.1174828267750273No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC840.11610067587179168No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA840.11610067587179168No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.11471852496855606No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT830.11471852496855606No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA820.11333637406532045No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA820.11333637406532045No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA800.11057207225884923No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT780.10780777045237801No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT760.10504346864590676No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.10504346864590676No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.10504346864590676No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC750.10366131774267114No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.10366131774267114No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG740.10227916683943554No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT730.10089701593619992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)