FastQCFastQC Report
Sun 21 May 2023
EGAF00007848262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65401
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG29714.542743994740142No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG29584.522866622834513No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21793.3317533371049377No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19272.946438127857372No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA13832.1146465650372317No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA12861.9663307900490818No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG10851.658996039815905No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG10551.6131251815721472No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9101.391416033393985No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8611.316493631595847No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT8561.308848488555221No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8451.292029173865843No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7841.1987584287702022No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7681.174293971040198No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7501.1467714560939435No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7441.137597284445192No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7171.0963135120258098No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6931.0596168254308038No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT6120.935765508172658No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT6040.9235332793076558No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6000.9174171648751548No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5940.9082429932264033No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5890.900597850185777No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5800.8868365927126497No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT5690.8700172780232718No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5100.7798045901438816No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4960.758398189630128No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT4860.7431079035488753No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT4790.7324047032919986No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4690.717114417210746No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT4150.6345468723719822No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4130.6314888151557315No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4090.6253727007232306No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3930.6009082429932264No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3870.5917340713444749No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT3860.5902050427363495No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3850.5886760141282243No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3840.587146985520099No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3710.5672696136144707No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3660.5596244705738445No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3490.533630984235715No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3390.5183406981544625No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3300.5045794406813351No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3250.49693429764070884No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3220.49234721181633306No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3170.4847020687757068No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3170.4847020687757068No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT3170.4847020687757068No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT3100.47399886851883No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2960.45259246800507635No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2960.45259246800507635No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.4480053821807006No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2920.44647635357257537No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2910.4449473249644501No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2890.4418892677481996No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2800.4281280102750722No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT2770.42354092445069647No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT2730.4174248100181954No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.41436675280194496No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2670.40825063836944386No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2540.38837326646381554No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2300.35167657986880935No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2290.3501475512606841No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2270.34708949404443357No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2260.3455604654363083No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCT2150.3287411507469305No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT2050.3134508646656779No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2030.3103928074494274No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.3103928074494274No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2000.3058057216250516No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1990.30427669301692634No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1950.2981605785844253No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1950.2981605785844253No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT1910.2920444641519243No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1880.2874573783275485No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.2736961208544212No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1780.2721670922462959No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1770.27063806363817067No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1750.26758000642192015No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.2645219492056696No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1730.2645219492056696No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCT1700.2599348633812939No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1680.2568768061650433No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1680.2568768061650433No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.25228972034066754No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCT1640.2507606917325423No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1640.2507606917325423No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1620.2477026345162918No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1580.24158652008379075No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.24158652008379075No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1560.23852846286754026No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1550.236999434259415No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1530.2339413770431645No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1530.2339413770431645No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1520.23241234843503922No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1520.23241234843503922No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCT1520.23241234843503922No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1500.2293542912187887No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1480.22629623400253818No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1470.22476720539441292No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1420.21712206235378664No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1340.20488983348878456No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1330.2033608048806593No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1330.2033608048806593No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1320.20183177627253407No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1320.20183177627253407No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1320.20183177627253407No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1310.2003027476644088No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1270.19418663323190777No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.18807051879940676No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1220.18654149019128147No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1200.18348343297503095No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1190.18195440436690571No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1170.1788963471506552No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1160.17736731854252993No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.1743092613262794No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.1743092613262794No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1130.17278023271815415No Hit
ATCTGTCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1130.17278023271815415No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.16972217550190366No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT1100.16819314689377837No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.16819314689377837No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCT1100.16819314689377837No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.16666411828565314No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1090.16666411828565314No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.16666411828565314No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.16513508967752785No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1080.16513508967752785No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCT1060.16207703246127733No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1060.16207703246127733No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1030.15748994663690158No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1020.15596091802877632No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1000.1529028608125258No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1000.1529028608125258No Hit
ATCTGTCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.14831577498815005No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC970.14831577498815005No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA960.14678674638002476No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT950.14525771777189952No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.142199660555649No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.142199660555649No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT910.13914160333939848No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT900.13761257473127322No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT900.13761257473127322No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT890.13608354612314796No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA890.13608354612314796No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.13302548890689744No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.13149646029877218No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT860.13149646029877218No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA860.13149646029877218No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA850.12996743169064695No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCT840.12843840308252166No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT840.12843840308252166No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA840.12843840308252166No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCT830.12690937447439643No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC830.12690937447439643No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA820.12538034586627114No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA810.1238513172581459No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT800.12232228865002065No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA770.11773520282564486No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT760.11620617421751961No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCT750.11467714560939435No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.11161908839314384No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT710.10856103117689332No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA700.10703200256876805No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA690.1055029739606428No Hit
ATCTGTCGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.10397394535251755No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.10244491674439228No Hit
ATCTGTCGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA660.10091588813626703No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC660.10091588813626703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)