FastQCFastQC Report
Sun 21 May 2023
EGAF00007848281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56173
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14662.6097947412457945No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12452.216367293895644No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11262.004521745322486No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9881.7588521175653782No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9381.6698413828707743No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8671.5434461396044363No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8561.5238637779716233No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8431.5007209869510263No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8181.4562156196037241No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7411.3191390881740337No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7341.3066775853167891No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7281.2959962971534367No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6531.1624801951115304No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6311.1233154718459046No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA6291.1197550424581204No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6161.0966122514375234No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6051.0770298898047104No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6021.0716892457230343No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5931.0556673134780055No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5851.0414255959268688No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5741.021843234294056No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA5600.9969202285795666No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5510.980898296334538No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5370.9559752906200487No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5250.9346127142933438No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5200.9257116408238835No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5170.920370996742207No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5030.895447991027718No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5020.893667776333826No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4980.8865469175582575No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4840.8616239118437683No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA4650.8277998326598188No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4630.8242394032720346No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4610.8206789738842504No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4540.8082174710270059No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4330.7708329624552721No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA4130.7352286685774304No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4080.72632759510797No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA4030.7174265216385096No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4010.7138660922507254No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3940.7014045893934808No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3930.6996243746995888No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3890.6925035159240205No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3880.6907233012301284No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3880.6907233012301284No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3730.6640200808217471No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3710.6604596514339629No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3600.64087728980115No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3510.6248553575561213No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3480.619514713474445No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3370.5999323518416321No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3230.5750093461271429No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3190.5678884873515746No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3050.5429654816370855No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2970.5287237640859488No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2880.51270183184092No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2840.5055809730653517No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2840.5055809730653517No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.5038007583714597No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2830.5038007583714597No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2730.4859986114325388No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2710.4824381820447546No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2620.46641624979972585No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2530.4503943175546971No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2510.446833888166913No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2380.4236910971463158No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2370.4219108824524238No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2320.41300980898296336No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2140.38096594449290583No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.38096594449290583No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2100.37384508571733754No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2020.3596033681662008No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.3560429387784167No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1980.3524825093906325No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1940.3453616506150642No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1920.34180122122728No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1890.33646057714560373No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.33646057714560373No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1820.32399907428835917No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1810.32221885959446706No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1750.3115375714311146No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1710.30441671265554626No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1650.29373542449219375No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1620.2883947804105175No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1580.28127392163494913No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.2705926334715967No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1500.2670322040838125No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.2670322040838125No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1420.25279048653267583No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.24032898367543123No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1250.22252683673651044No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1240.22074662204261833No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC1190.2118455485731579No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.20828511918537373No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1160.20650490449148168No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.20294447510369754No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1140.20294447510369754No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.1993840457159133No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1100.19582361632812917No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.19582361632812917No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1090.19404340163423708No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1080.192263186940345No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT1070.19048297224645291No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1060.18870275755256083No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT1050.18692254285866877No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT1040.1851423281647767No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1030.1833621134708846No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1020.1815818987769925No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC990.17624125469531626No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT970.1726808253075321No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC960.17090061061364No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA940.16734018122585584No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.16734018122585584No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC920.16377975183807167No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT900.1602193224502875No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.1602193224502875No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.15665889306250336No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC870.15487867836861124No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA870.15487867836861124No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT870.15487867836861124No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT860.15309846367471916No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.15309846367471916No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT860.15309846367471916No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC840.149538034286935No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA830.14775781959304293No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT820.14597760489915085No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.14597760489915085No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT800.14241717551136668No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT790.14063696081747457No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.1388567461235825No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA770.13707653142969042No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC770.13707653142969042No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA770.13707653142969042No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT770.13707653142969042No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT760.13529631673579834No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC760.13529631673579834No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA740.13173588734801417No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT730.12995567265412208No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG710.12639524326633791No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT710.12639524326633791No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.12639524326633791No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA700.12461502857244583No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC690.12283481387855374No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT680.12105459918466167No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT680.12105459918466167No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.12105459918466167No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACACT660.1174941697968775No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT660.1174941697968775No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC640.11393374040909332No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC640.11393374040909332No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA630.11215352571520125No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG630.11215352571520125No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC620.11037331102130916No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACT610.10859309632741708No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT610.10859309632741708No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT610.10859309632741708No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.10681288163352501No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCC600.10681288163352501No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.10503266693963292No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCC590.10503266693963292No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG590.10503266693963292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)