FastQCFastQC Report
Sun 21 May 2023
EGAF00007848284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74483
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA33924.554059315548514No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA33574.507068727092088No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA23233.118832485265094No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG22413.008740249452895No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22222.9832310728622637No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG20502.752305895304969No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT16652.2354094222842797No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG15602.0944376569150007No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG14451.9400400091296No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13181.7695313024448531No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11411.5318931836794973No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT11211.5050414188472538No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT10121.358699300511526No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10011.343930829853792No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9751.3090235355718756No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9511.2768014177731832No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9261.2432367117328786No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT9181.2324960057999812No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9121.224440476350308No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8581.1519407113032505No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7731.037820710766215No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT7471.0029134164842985No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7400.9935152987930131No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT7160.9612931809943208No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7040.9451821220949748No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7020.9424969456117503No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6570.8820804747392023No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT6420.8619416511150194No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6250.8391176510076124No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT6060.8136084744169811No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6050.8122658861753689No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5920.7948122390344106No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT5860.7867567095847374No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5470.7343957681618625No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT5360.7196272975041285No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5270.7075440033296189No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5110.686062591463824No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5070.6806922384973753No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4720.6337016500409489No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4670.626988708832888No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4660.6256461205912759No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT4450.5974517675174201No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4340.5826832968596861No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4270.5732851791684008No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4190.5625444732355034No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3970.5330075319200355No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3870.5195816495039136No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3860.5182390612623015No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3800.5101835318126284No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3580.4806465904971604No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3450.46319294335620215No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3340.4484244726984681No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3310.44439670797363157No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3270.43902635500718284No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3220.4323134137991219No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3170.42560047259106104No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3160.4242578843494489No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3120.41888753138300017No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3060.4108320019333271No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2950.39606353127559313No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2880.38666541358430784No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2880.38666541358430784No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2770.3718969429265738No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2740.3678691782017373No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2680.35981364875206423No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2480.3329618839198206No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2450.3289341191949841No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2250.3020823543627405No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2190.2940268249130674No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.2926842366714552No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2120.28462870722178213No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2110.28328611898017No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2090.2806009424969456No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2080.2792583542553334No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1980.2658324718392116No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1910.25643435414792637No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1820.24435105997341675No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1800.2416658834901924No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1780.23898070700696805No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1750.23495294228213148No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1700.2282400010740706No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1700.2282400010740706No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.22421223634923404No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1660.2228696481076219No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.21884188338278532No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1620.21749929514117317No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1560.20944376569150008No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1520.20407341272505136No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1510.20273082448343918No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1420.19064753030892956No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1360.18259200085925648No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1330.17856423613441993No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.1745364714095834No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1300.1745364714095834No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1290.1731938831679712No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1270.17050870668474685No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1270.17050870668474685No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1260.16916611844313467No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.16916611844313467No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.16916611844313467No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1230.16513835371829816No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1220.16379576547668598No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1180.15842541251023723No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1150.1543976477854007No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.14499953009411543No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1070.14365694185250324No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1050.14097176536927888No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1040.1396291771276667No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1030.13828658888605452No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.13560141240283016No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1000.13425882416121798No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.13425882416121798No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT990.1329162359196058No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.1275458829531571No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC930.12486070646993273No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC920.12351811822832055No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT920.12351811822832055No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.1208329417450962No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.11546258877864748No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA840.11277741229542312No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC840.11277741229542312No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC840.11277741229542312No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA820.11009223581219876No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.11009223581219876No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA800.1074070593289744No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA800.1074070593289744No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC790.10606447108736222No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.10606447108736222No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.10337929460413786No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA760.10203670636252568No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA760.10203670636252568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)