FastQCFastQC Report
Sun 21 May 2023
EGAF00007848293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72876
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG23333.2013282836599153No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT19602.689499972556123No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG18632.5563971677918658No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG17732.432899720072452No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG14982.0555464075964656No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13871.9032328887425214No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12791.7550359514792249No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11281.5478346780833196No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10511.4421757505900432No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA10331.4174762610461604No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10221.4023821285471212No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9371.2857456501454525No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9071.2445798342389813No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA9051.2418354465118833No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8851.2143915692409024No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA8751.2006696306054119No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8721.196553049014765No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA8151.1183379987924693No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA8001.0977550908392337No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7831.0744277951589No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7641.0483561117514684No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA7200.9879795817553103No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7140.9797464185740161No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA7040.9660244799385258No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6820.9358362149404468No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6570.9015313683517207No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6390.876831878807838No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6200.8507601954004061No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6150.843899226082661No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA5980.8205719304023272No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT5880.8068499917668368No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5510.7560788188155223No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT5460.749217849497777No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5430.7451012679071299No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5150.7066798397277567No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5120.7025632581371096No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5070.6957022888193644No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4890.6710027992754817No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4870.6682584115483835No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4770.6545364729128932No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4720.6476755035951479No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT4670.6408145342774026No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4530.6216038201877161No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4440.6092540754157747No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4320.5927877490531862No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4270.585926779735441No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4250.583182392008343No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4210.5776936165541467No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3860.5296668313299303No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3760.5159448926944399No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3750.5145726988308909No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3680.5049673417860475No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3640.4994785663318514No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT3640.4994785663318514No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3570.48987320928700806No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3530.4843844338328119No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3470.47615127065151763No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3430.4706624951973215No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3420.4692903013337724No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3370.4624293320160272No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3250.4459630056534387No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3150.4322410670179483No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3000.4116581590647127No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2990.4102859652011636No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT2950.40479718974696743No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2940.4034249958834184No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2920.40068060815632034No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2860.39244744497502604No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.38833086338437894No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2820.3869586695208299No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2800.38421428179373185No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2780.38146989406663373No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT2780.38146989406663373No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT2640.3622591799769471No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2640.3622591799769471No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2470.3389318842966134No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2450.33618749656951535No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT2440.3348153027059663No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2420.3320709149788682No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2340.3210933640704759No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2300.3156045886162797No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT2240.30737142543498547No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2110.28953290520884795No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.28953290520884795No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.2854163236182008No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.2799275481640046No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.2785553543004556No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.27169438498271037No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1970.2703221911191613No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1890.259344640210769No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.25111147702947473No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1790.24562270157527855No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT1760.24150611998463145No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1730.2373895383939843No Hit
TGAATATCGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.23601734453043527No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1720.23601734453043527No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1710.2346451506668862No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1710.2346451506668862No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1630.22366759975849387No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT1630.22366759975849387No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1610.22092321203139578No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1580.2168066304407487No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1550.21269004885010154No Hit
TGAATATCGCCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT1500.20582907953235635No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT1460.20034030407816017No Hit
TGAATATCGCCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT1460.20034030407816017No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1410.19347933476041496No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1390.19073494703331687No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1330.18250178385202262No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1330.18250178385202262No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1310.17975739612492453No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1300.1783852022613755No Hit
TGAATATCGCCGGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCT1290.17701300839782644No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1270.17426862067072837No Hit
TGAATATCGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1270.17426862067072837No Hit
TGAATATCGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1240.17015203908008122No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1240.17015203908008122No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1150.15780229430813986No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT1140.15643010044459082No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1120.15368571271749273No Hit
TGAATATCGCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT1110.15231351885394367No Hit
TGAATATCGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1100.15094132499039464No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1060.14545254953619846No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1050.14408035567264943No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.14408035567264943No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT1050.14408035567264943No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1040.1427081618091004No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1010.13859158021845328No Hit
TGAATATCGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT1000.13721938635490422No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.13721938635490422No Hit
TGAATATCGCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT990.13584719249135518No Hit
TGAATATCGCCGGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCT980.13447499862780612No Hit
AAGGACGGCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG930.1276140293100609No Hit
TGAATATCGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC930.1276140293100609No Hit
TGAATATCGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC920.12624183544651188No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG910.12486964158296285No Hit
TGAATATCGCCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT900.12349744771941379No Hit
TGAATATCGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT900.12349744771941379No Hit
TGAATATCGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC890.12212525385586474No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACT880.12075305999231573No Hit
TGAATATCGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC880.12075305999231573No Hit
TGAATATCGCCGGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCT880.12075305999231573No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA860.11800867226521763No Hit
TGAATATCGCCGGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCT840.11526428453811956No Hit
TGAATATCGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.11389209067457051No Hit
TGAATATCGCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT820.11251989681102147No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA800.10977550908392336No Hit
AAGGACGGCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.10840331522037434No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.1070311213568253No Hit
TGAATATCGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC770.10565892749327624No Hit
AAGGACGGCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA770.10565892749327624No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA760.1042867336297272No Hit
TGAATATCGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT750.10291453976617818No Hit
AAGGACGGCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA750.10291453976617818No Hit
TGAATATCGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC730.10017015203908008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)