FastQCFastQC Report
Sun 21 May 2023
EGAF00007848308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55759
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16923.0344877060205526No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16452.950196380853315No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13072.3440162126293513No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG12712.279452644416148No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10621.9046252622894957No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10481.8795172079843614No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10271.8418551265266596No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9611.7234885848024535No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8941.6033286106278806No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8441.5136569881095427No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8221.4742014742014742No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7991.4329525278430388No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA7821.402464176186804No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7761.3917035814846035No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7571.3576283649276348No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7511.3468677702254346No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7351.3181728510195663No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6911.2392618232034291No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6761.2123603364479276No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6751.2105669039975608No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6751.2105669039975608No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6481.1621442278376586No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5410.9702469556484156No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5300.9505191986943811No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5230.9379651715418139No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5230.9379651715418139No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5130.9200308470381464No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5040.9038899549848455No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5020.9003030900841119No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5020.9003030900841119No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4990.8949227927330118No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4850.8698147384278772No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4620.8285657920694417No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4490.8052511702146739No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4370.7837299808102728No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4280.767589088756972No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4140.7424810344518373No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4100.7353073046503703No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4020.7209598450474363No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3750.6725371688875339No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3530.6330816549794652No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3510.6294947900787317No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.6097670331246974No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3370.604386735773597No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3300.5918327086210298No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3260.5846589788195627No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3190.5721049516669955No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.5344428702092936No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.5344428702092936No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2970.5326494377589268No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2940.5272691404078265No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2830.5075413834537922No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2770.4967807887515917No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.4860201940493912No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2670.4788464642479241No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2610.46808586954572357No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2580.46270557219462327No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2580.46270557219462327No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2490.4465646801413225No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2440.43759751788948864No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2380.4268369231872881No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2360.42325005828655465No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2220.3981420039814201No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2210.3963485715310533No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2170.3891748417295862No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2160.3873814092792195No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2160.3873814092792195No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2150.38558797682885276No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2070.37124051722591866No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2060.36944708477555194No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2020.3622733549740849No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1940.3479258953711509No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1800.32281784106601624No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1770.317437543714916No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1760.3156441112645492No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1740.3120572463638157No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.3048835165623487No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1670.29950321921124845No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1580.28336232715794757No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.28156889470758084No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.27260173245574704No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1450.2600477053031798No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1400.251080543051346No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1290.23135278609731164No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1270.22776592119657812No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.22597248874621137No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1240.22238562384547786No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1220.21879875894474432No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1180.21162502914327733No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1170.20983159669291057No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1160.2080381642425438No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1150.20624473179217703No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1130.20265786689144355No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.20086443444107677No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1120.20086443444107677No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.19907100199071004No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1060.19010383973887623No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.18831040728850948No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.18831040728850948No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1030.18472354238777597No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1020.1829301099374092No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1020.1829301099374092No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1020.1829301099374092No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1010.18113667748704246No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1000.17934324503667567No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1000.17934324503667567No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC970.17396294768557544No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA960.17216951523520868No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC950.1703760827848419No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA950.1703760827848419No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT950.1703760827848419No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT940.16858265033447517No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.1667892178841084No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA930.1667892178841084No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA930.1667892178841084No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA910.16320235298337488No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC890.15961548808264137No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.15602862318190786No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.15602862318190786No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.15064832583080756No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA840.15064832583080756No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC820.14706146093007405No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG810.14526802847970732No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA810.14526802847970732No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT780.13988773112860706No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA780.13988773112860706No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC740.13271400132714No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT730.13092056887677325No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA710.12733370397603974No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC710.12733370397603974No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.12195340662493946No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA670.12015997417457272No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.12015997417457272No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA670.12015997417457272No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC670.12015997417457272No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG670.12015997417457272No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC670.12015997417457272No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC660.11836654172420596No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.11477967682347245No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA600.10760594702200542No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA600.10760594702200542No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT600.10760594702200542No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC590.10581251457163866No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.10581251457163866No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC590.10581251457163866No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.1040190821212719No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.10222564967090515No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.10043221722053838No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC560.10043221722053838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)