FastQCFastQC Report
Sun 21 May 2023
EGAF00007848309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55759
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16652.9860650298606504No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15472.774440000717373No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG11812.1180437238831398No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11812.1180437238831398No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10511.8848975053354615No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9811.7593572338097885No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9781.7539769364586881No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9271.6625118814899835No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9161.6427841245359496No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8921.599741745727147No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8911.5979483132767802No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8821.5818074212234796No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8681.5566993669183449No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7801.3988773112860704No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7381.3235531483706666No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6971.2500224179056296No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6911.2392618232034291No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6561.1764916874405924No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6541.172904822539859No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6391.1460033357843578No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6331.135242741082157No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6221.1155149841281229No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6041.0832332000215212No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5791.0383973887623523No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5601.004322172205384No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5410.9702469556484156No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5180.92899800928998No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5060.9074768198855789No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4950.8877490629315447No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4890.8769884682293441No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4830.8662278735271436No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4820.8644344410767769No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4740.8500869814738428No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4580.8213920622679748No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4500.8070446026650406No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4290.7693825212073387No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4250.7622087914058717No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4170.7478613318029377No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4050.7263401423985366No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3840.6886780609408347No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3830.6868846284904679No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3790.6797108986890008No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3730.6689503039868003No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3450.6187341953765312No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3450.6187341953765312No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3450.6187341953765312No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3220.5774852490180957No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3170.5685180867662619No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2990.5362363026596604No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2980.5344428702092936No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2890.5183019781559928No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2870.5147151132552592No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.5075413834537922No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2650.47525959934719053No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2570.4609121397442566No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2500.4483581125916893No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2370.4250434907369214No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2350.4214566258361879No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.41966319338582114No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2330.41786976093545436No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.41428289603472085No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2180.390968274179953No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2180.390968274179953No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2100.37662081457701896No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2020.3622733549740849No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2010.36047992252371813No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2010.36047992252371813No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1930.3461324629207841No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1900.3407521655696838No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1860.3335784357682168No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1850.33178500331785005No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.33178500331785005No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.32281784106601624No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1740.3120572463638157No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1730.3102638139134489No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1730.3102638139134489No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1680.3012966516616151No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1640.2941229218601481No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.28874262450904786No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1590.28515575960831435No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.27618859735648055No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1530.2743951649061138No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1510.2708083000053803No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1380.24749367815061246No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1340.24031994834914544No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.24031994834914544No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.23493965099804517No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.22776592119657812No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1220.21879875894474432No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1210.2170053264943776No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1210.2170053264943776No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1200.21521189404401084No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1160.2080381642425438No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1140.2044512993418103No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1130.20265786689144355No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1130.20265786689144355No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1120.20086443444107677No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1110.19907100199071004No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1100.19727756954034328No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1090.1954841370899765No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1070.191897272189243No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.191897272189243No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.18831040728850948No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1050.18831040728850948No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.18651697483814272No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.18113667748704246No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.17934324503667567No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA980.17575638013594216No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC980.17575638013594216No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT970.17396294768557544No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC960.17216951523520868No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA950.1703760827848419No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA950.1703760827848419No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA940.16858265033447517No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC930.1667892178841084No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC870.15602862318190786No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.15602862318190786No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA870.15602862318190786No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA870.15602862318190786No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA860.1542351907315411No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA850.15244175828117434No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.15244175828117434No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT840.15064832583080756No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.14885489338044083No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA830.14885489338044083No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA820.14706146093007405No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.14706146093007405No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.14526802847970732No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.14347459602934057No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC790.14168116357897378No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC780.13988773112860706No Hit
TAGCCAATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG710.12733370397603974No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC690.12374683907530623No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC690.12374683907530623No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.12195340662493946No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA660.11836654172420596No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC630.11298624437310568No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC630.11298624437310568No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG620.11119281192273893No Hit
TAGCCAATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.11119281192273893No Hit
TAGCCAATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA620.11119281192273893No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.10939937947237216No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.10939937947237216No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC600.10760594702200542No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.10760594702200542No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC600.10760594702200542No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.1040190821212719No Hit
TAGCCAATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA580.1040190821212719No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.10222564967090515No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT570.10222564967090515No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC570.10222564967090515No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC570.10222564967090515No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.10043221722053838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)