FastQCFastQC Report
Sun 21 May 2023
EGAF00007848310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13853
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8396.056449866454919No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5113.6887316826680143No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4723.4072042156933517No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4633.342236338699199No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4112.966866382732982No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2822.0356601458167907No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2601.8768497798310837No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2541.8335378618349816No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2411.7396953728434275No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2211.5953223128564211No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2181.57366635385837No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2111.5231357828629177No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2101.5159171298635674No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2081.5014798238648668No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2051.4798238648668158No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1991.436511946870714No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1861.3426694578791598No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1851.3354508048798095No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1761.2704829278856566No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1741.256045621886956No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1731.2488269688876055No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1721.2416083158882552No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1711.2343896628889048No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1641.1838590918934526No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1611.1622031328954017No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1461.053923337905147No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1350.9745181549122933No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.9312062369161914No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1280.9239875839168412No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1280.9239875839168412No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1180.8518010539233379No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1100.7940518299285354No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1100.7940518299285354No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1060.765177217931134No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1060.765177217931134No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.7579585649317837No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1020.7363026059337328No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.7218652999350321No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT960.6929906879376309No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.6857720349382805No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG950.6857720349382805No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT930.6713347289395799No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT910.6568974229408793No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT880.6352414639428283No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA840.6063668519454269No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA830.5991481989460766No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA800.5774922399480257No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA750.5413989749512741No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT750.5413989749512741No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT720.5197430159532231No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT690.4980870569551722No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.4547751389590703No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT610.4403378329603696No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT590.42590052696166897No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC580.41868187396231865No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG520.3753699559662167No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA500.36093264996751606No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT490.35371399696816574No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG490.35371399696816574No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA480.34649534396881543No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA470.3392766909694651No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT470.3392766909694651No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT470.3392766909694651No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT460.3320580379701148No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT460.3320580379701148No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.3248393849707645No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG440.31762073197141416No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA420.30318342597271347No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT410.29596477297336315No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA400.28874611997401284No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA380.2743088139753122No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC380.2743088139753122No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT360.25987150797661157No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA360.25987150797661157No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT350.25265285497726125No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC350.25265285497726125No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT350.25265285497726125No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC340.24543420197791094No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT330.23821554897856062No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC320.2309968959792103No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA320.2309968959792103No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC310.22377824297985996No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA300.21655958998050964No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA300.21655958998050964No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA280.202122283981809No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT280.202122283981809No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA280.202122283981809No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA280.202122283981809No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT270.19490363098245866No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT270.19490363098245866No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA270.19490363098245866No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA260.18768497798310835No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC260.18768497798310835No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA250.18046632498375803No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT250.18046632498375803No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT240.17324767198440771No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA220.15881036598570708No Hit
AATCTGACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT220.15881036598570708No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT220.15881036598570708No Hit
AATCTGACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT220.15881036598570708No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC220.15881036598570708No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC220.15881036598570708No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA210.15159171298635674No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT200.14437305998700642No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC200.14437305998700642No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA200.14437305998700642No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT190.1371544069876561No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC180.12993575398830579No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA180.12993575398830579No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA170.12271710098895547No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC170.12271710098895547No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT170.12271710098895547No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT170.12271710098895547No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT170.12271710098895547No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA170.12271710098895547No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA160.11549844798960515No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT160.11549844798960515No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC160.11549844798960515No Hit
AATCTGACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT160.11549844798960515No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT160.11549844798960515No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA150.10827979499025482No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG150.10827979499025482No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT140.1010611419909045No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC140.1010611419909045No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA140.1010611419909045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)