FastQCFastQC Report
Sun 21 May 2023
EGAF00007848318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75099
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA31404.181147551898161No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA29323.904179815976245No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21722.892182319338473No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20272.699103849585214No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT13131.7483588330070972No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA10541.4034807387581725No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT10541.4034807387581725No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10021.3342388047776934No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9741.2969546864805124No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9341.2436916603416823No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9341.2436916603416823No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9331.2423600846882117No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9181.2223864498861503No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT8921.1877654828959108No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8191.0905604601925458No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7991.0639289471231308No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7921.0546079175488354No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7921.0546079175488354No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT7771.0346342827467743No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7551.0053396183704177No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7210.9600660461524122No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6880.9161240495878773No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6760.9001451417462283No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6580.8761767799837548No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6570.8748452043302841No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6070.8082664216567463No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6030.8029401190428634No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5820.7749770303199777No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT5690.7576665468248579No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5520.735029760715855No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5440.7243771554880891No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5310.7070666719929692No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT5090.6777720076166127No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5050.6724457050027297No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4920.6551352215076099No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4900.6524720702006684No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4820.6418194649729024No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4700.6258405571312534No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4700.6258405571312534No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4700.6258405571312534No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4570.6085300736361337No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4500.5992090440618384No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4450.5925511657944846No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4230.5632565014181281No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.5579301988042451No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4170.5552670474973036No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4130.5499407448834206No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4070.541951290962596No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3880.5166513535466518No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3840.5113250509327687No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3750.499340870051532No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3740.49800929439806124No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3710.49401456743764893No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA3690.49135141613070743No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3580.47670408394252917No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3490.4647199030612924No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3450.45939360044740946No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3440.45806202479393865No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3310.4407515412988189No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3290.4380883899918774No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA3230.4300989360710529No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3220.4287673604175821No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3150.41944633084328686No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3130.41678317953634536No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3130.41678317953634536No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2970.39547796908081334No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2920.3888200908134596No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2860.380830636892635No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2840.3781674855856935No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2660.35419912382322No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2640.35153597251627855No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2620.348872821209337No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2510.33422548902115873No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2510.33422548902115873No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2500.332893913367688No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2410.3209097324864512No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2330.3102571272586852No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2290.3049308246448022No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2290.3049308246448022No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2280.30359924899133145No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2250.2996045220309192No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA2250.2996045220309192No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2240.29827294637744844No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.2902834924566239No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2080.27696773592191637No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2060.2743045846149749No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1980.26365197938720886No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1900.25299937415944285No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1870.24900464719903062No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1860.24767307154555987No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTA1860.24767307154555987No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1850.24634149589208912No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1840.24500992023861837No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1840.24500992023861837No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.24367834458514762No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1820.24234676893167684No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.24101519327820609No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1790.23835204197126458No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTA1770.2356888906643231No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1740.23169416370391083No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.23036258805044008No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1700.22636786109002782No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1590.21172052890184953No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1560.2077258019414373No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1560.2077258019414373No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.20373107498102505No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1530.20373107498102505No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1420.18908374279284676No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1410.18775216713937604No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1400.18642059148590528No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1400.18642059148590528No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1340.17843113756508075No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1300.17310483495119774No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1290.171773259297727No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1290.171773259297727No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1270.16911010799078552No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1240.16511538103037324No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1230.16378380537690249No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1220.16245222972343173No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1210.16112065406996098No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1210.16112065406996098No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTA1210.16112065406996098No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.15845750276301948No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1190.15845750276301948No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1170.15579435145607798No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTA1140.15179962449566572No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1110.14780489753525347No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1080.14381017057484122No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1070.14247859492137047No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1070.14247859492137047No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.13981544361442894No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.13715229230748746No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1030.13715229230748746No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1020.1358207166540167No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.1358207166540167No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1010.13448914100054596No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1000.13315756534707518No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA980.1304944140401337No Hit
AAGAGTATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA930.12383653577277993No Hit
AAGAGTATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA920.12250496011930918No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT920.12250496011930918No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT910.12117338446583842No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG910.12117338446583842No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT900.11984180881236767No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC900.11984180881236767No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.11851023315889693No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT880.11717865750542618No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC870.11584708185195541No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.11584708185195541No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.11584708185195541No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT870.11584708185195541No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG860.11451550619848466No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC850.11318393054501391No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.11185235489154317No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG840.11185235489154317No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT830.11052077923807242No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT830.11052077923807242No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC820.10918920358460166No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT810.1078576279311309No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.10652605227766014No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT800.10652605227766014No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.10519447662418942No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT790.10519447662418942No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC790.10519447662418942No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC780.10386290097071865No Hit
AAGAGTATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA780.10386290097071865No Hit
AAGAGTATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG770.1025313253172479No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT760.10119974966377715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)