FastQCFastQC Report
Sun 21 May 2023
EGAF00007848320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31750
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG10463.294488188976378No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG10283.2377952755905515No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9172.8881889763779527No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8502.677165354330709No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6652.094488188976378No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6632.0881889763779524No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4661.4677165354330708No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4561.4362204724409449No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4501.4173228346456692No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4421.3921259842519684No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4361.373228346456693No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4271.3448818897637795No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3991.2566929133858267No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3871.2188976377952756No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3761.184251968503937No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3541.1149606299212598No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3481.0960629921259841No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3471.0929133858267717No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA3441.0834645669291338No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3391.0677165354330709No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3341.051968503937008No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA3231.0173228346456693No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3090.973228346456693No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3060.9637795275590552No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2990.9417322834645669No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2970.9354330708661417No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.9291338582677164No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2900.9133858267716535No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2770.8724409448818897No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2700.8503937007874015No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.8220472440944883No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2430.7653543307086614No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2390.752755905511811No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2260.7118110236220473No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2110.6645669291338583No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2100.6614173228346456No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2080.6551181102362205No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2050.6456692913385828No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1830.5763779527559054No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.5637795275590551No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1770.5574803149606299No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1740.5480314960629921No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1730.5448818897637795No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.525984251968504No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1560.4913385826771654No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.4881889763779528No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.4850393700787402No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.46929133858267713No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.4598425196850393No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1460.4598425196850393No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1430.4503937007874016No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1430.4503937007874016No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1420.447244094488189No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1380.4346456692913386No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1360.4283464566929134No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.4283464566929134No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1350.42519685039370075No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1330.41889763779527556No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1330.41889763779527556No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1310.4125984251968504No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1310.4125984251968504No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.4125984251968504No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1280.4031496062992126No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.3968503937007874No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1230.3874015748031496No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1220.384251968503937No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1220.384251968503937No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1180.3716535433070866No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.368503937007874No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1120.352755905511811No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.33700787401574805No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1070.33700787401574805No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT980.30866141732283464No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA970.30551181102362207No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT950.2992125984251969No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC940.29606299212598425No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA920.28976377952755905No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA890.2803149606299213No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT870.27401574803149603No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC870.27401574803149603No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA860.27086614173228346No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA850.26771653543307083No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA840.26456692913385826No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT800.25196850393700787No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT800.25196850393700787No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG740.23307086614173228No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.22992125984251965No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC720.2267716535433071No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT710.2236220472440945No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA710.2236220472440945No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.2204724409448819No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT690.2173228346456693No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.2173228346456693No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC680.2141732283464567No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT680.2141732283464567No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTG670.2110236220472441No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.2110236220472441No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT660.20787401574803152No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT640.2015748031496063No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.2015748031496063No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA640.2015748031496063No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.1952755905511811No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT620.1952755905511811No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.1921259842519685No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC610.1921259842519685No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT610.1921259842519685No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC610.1921259842519685No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC600.1889763779527559No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT590.1858267716535433No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.1858267716535433No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA580.1826771653543307No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT570.1795275590551181No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA550.1732283464566929No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA550.1732283464566929No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA550.1732283464566929No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1700787401574803No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC540.1700787401574803No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1700787401574803No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTG520.16377952755905512No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG520.16377952755905512No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC510.16062992125984252No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC500.15748031496062992No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT480.15118110236220472No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC480.15118110236220472No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC470.14803149606299212No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT470.14803149606299212No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA470.14803149606299212No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA460.14488188976377953No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC460.14488188976377953No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT450.14173228346456693No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA450.14173228346456693No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA450.14173228346456693No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA440.13858267716535433No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA430.13543307086614173No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT420.13228346456692913No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.13228346456692913No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC420.13228346456692913No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA420.13228346456692913No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA400.12598425196850394No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA390.12283464566929135No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA390.12283464566929135No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC390.12283464566929135No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCT390.12283464566929135No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC380.11968503937007874No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA370.11653543307086614No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.11653543307086614No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.11653543307086614No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA370.11653543307086614No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT370.11653543307086614No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC360.11338582677165356No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG350.11023622047244094No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC350.11023622047244094No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA350.11023622047244094No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA350.11023622047244094No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT340.10708661417322834No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.10393700787401576No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCT330.10393700787401576No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA330.10393700787401576No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC330.10393700787401576No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA330.10393700787401576No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA320.10078740157480315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)