FastQCFastQC Report
Sun 21 May 2023
EGAF00007848326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52726
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20693.924060235936729No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA17013.2261123544361414No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15642.9662784963774986No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA12472.3650570875848724No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11762.230398664795357No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA9871.8719417365246749No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9261.7562492887759358No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA8961.6993513636536055No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8881.684178583620984No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA8271.5684861358722453No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8091.534347380798847No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7921.5021052232295262No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7631.4471038956112734No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6911.3105488753176802No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6691.268823730227971No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6411.2157190001137959No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6341.202442817585252No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5911.1208891249099118No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5731.0867503698365133No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5711.082957174828358No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5541.0507150172590372No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5511.0450252247468044No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5371.0184728596897166No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5321.0089898721693282No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5040.9558851420551531No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4920.9331259720062209No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4910.931229374502143No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4680.8876076319083563No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4590.8705382543716573No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4220.8003641467207828No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4140.7851913666881615No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4010.7605355991351515No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3980.7548458066229184No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3960.7510526116147631No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3940.7472594166066078No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3920.7434662215984523No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3890.7377764290862193No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3860.7320866365739862No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3860.7320866365739862No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA3730.7074308690209764No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3730.7074308690209764No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3410.6467397488904905No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3300.625877176345636No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3240.6144975913211699No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3210.6088077988089368No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3150.5974282137844706No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3150.5974282137844706No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3060.5803588362477715No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3010.5708758487273831No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.5689792512233054No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.559496263702917No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2910.5519098736866063No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2780.5272541061335964No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2760.5234609111254409No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2680.5082881310928195No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.5044949360846641No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2650.5025983385805864No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2640.5007017410765088No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2620.4969085460683534No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2620.4969085460683534No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2610.4950119485642757No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2530.4798391685316542No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2530.4798391685316542No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2530.4798391685316542No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2510.4760459735234988No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2500.47414937601942114No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2410.457079998482722No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2400.4551834009786443No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA2330.4419072184501005No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2310.4381140234419451No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2130.4039752683685468No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2120.4020786708644692No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2080.39449228084815835No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA2050.38880248833592534No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2040.3869058908318477No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1990.3774229033114592No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1960.37173311079922616No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.3508705382543717No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1830.34707734324621625No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1800.34138755073398325No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.3281113682054394No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.3224215756932064No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.318628380685051No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1680.318628380685051No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1590.3015590031483519No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1540.2920760156279634No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1470.27879983309941964No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1340.2541440655464097No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1290.24466107802602133No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1290.24466107802602133No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1280.24276448052194363No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1250.23707468800971057No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.23328149300155523No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1200.22759170048932215No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1040.19724614042407917No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.19534954292000153No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1010.19155634791184614No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1000.18965975040776847No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA990.18776315290369078No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA940.17828016538330235No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA930.17638356787922466No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA910.17259037287106932No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT900.17069377536699162No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT890.16879717786291393No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT850.1612107878466032No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC850.1612107878466032No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT830.1574175928384478No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC810.15362439783029247No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.14414141030990404No Hit
TCCTAACAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA740.14034821530174865No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA700.13276182528543792No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC690.13086522778136023No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA680.12896863027728256No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC680.12896863027728256No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.12896863027728256No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG670.12707203277320486No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA670.12707203277320486No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC670.12707203277320486No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC660.1251754352691272No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC650.1232788377650495No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.11948564275689413No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT630.11948564275689413No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT610.11569244774873877No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA610.11569244774873877No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA590.11189925274058339No Hit
TCCTAACAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA580.11000265523650572No Hit
TCCTAACAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA560.10620946022835034No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.10620946022835034No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.10620946022835034No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC550.10431286272427265No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC540.10241626522019497No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC540.10241626522019497No Hit
TCCTAACAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA530.1005196677161173No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.1005196677161173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)