FastQCFastQC Report
Sun 21 May 2023
EGAF00007848336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57068
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA29305.134225835844957No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19923.4905726501717247No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15952.79491133384734No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11221.966075558982267No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA11081.9415434218826664No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA10631.862690124062522No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9691.6979743463937758No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9381.6436531856732317No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA8741.5315062732179154No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8731.5297539777108011No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8581.5034695451040863No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT8451.4806897035116002No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT7791.3650382000420551No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7771.3615336090278265No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7711.3510198359851404No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7411.298450970771711No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7391.2949463797574823No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6621.1600196257096798No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6461.1319828975958506No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6381.117964533538936No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6061.0618910773112777No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA6041.0583864862970491No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5440.95324875587019No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5230.9164505502207894No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5180.9076890726852176No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5100.8936707086283031No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4810.8428541389219878No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4810.8428541389219878No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4800.8411018434148735No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4600.8060559332725872No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA4530.7937898647227869No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4420.7745146141445294No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4390.7692577276231863No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4350.7622485455947291No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4210.7377164084951285No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4140.7254503399453284No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4110.7201934534239853No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT4110.7201934534239853No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3960.6939090208172706No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3940.690404429803042No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3800.6658722927034415No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3740.6553585196607555No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3570.6255694960398122No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3490.6115511319828977No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3380.59227588140464No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3380.59227588140464No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA3340.5852666993761828No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3330.5835144038690685No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3320.5817621083619541No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3240.5677437443050396No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.5432116072054392No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT3030.5309455386556389No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2950.5169271745987243No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2910.509917992570267No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2790.4888904464848952No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2740.4801289689493236No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2650.46435830938529477No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2650.46435830938529477No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2620.4591014228639518No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2460.43106469475012266No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2450.42931239924300835No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2400.42055092170743674No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2380.4170463306932081No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.41003714866475083No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.37499123852246447No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2100.36798205649400717No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.35045910142286396No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.3469545104086353No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.34344991939440667No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1890.33118385084460644No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1850.32417466881614915No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1790.31366089577346323No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1760.30840400925212025No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1740.30489941823789163No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1730.3031471227307773No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1720.301394827223663No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.2943856451952057No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1510.2645966215742623No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.257587439545805No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1470.257587439545805No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.25233055302446206No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1370.2400644844746618No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1370.2400644844746618No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1370.2400644844746618No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1350.23655989346043318No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1340.23480759795331882No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1330.2330553024462045No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1310.2295507114319759No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1280.22429382491063293No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.21903693838928998No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1250.21903693838928998No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1180.20677086983948972No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1160.2032662788252611No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC1150.2015139833181468No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1070.18749561926123223No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.18399102824700359No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.18223873273988925No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.18223873273988925No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1020.17873414172566063No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1010.1769818462185463No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1000.17522955071143198No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1000.17522955071143198No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA970.16997266419008902No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT970.16997266419008902No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA960.16822036868297469No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC950.16646807317586038No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT940.16471577766874607No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.16121118665451742No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT920.16121118665451742No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT900.15770659564028877No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC900.15770659564028877No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT890.15595430013317446No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT880.15420200462606012No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA880.15420200462606012No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.15244970911894581No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA850.14894511810471717No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT840.14719282259760286No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT830.14544052709048852No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1436882315833742No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG820.1436882315833742No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.1436882315833742No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC790.13843134506203125No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC780.13667904955491694No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.13667904955491694No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC780.13667904955491694No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA770.1349267540478026No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.1349267540478026No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.1331744585406883No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC760.1331744585406883No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.13142216303357399No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA750.13142216303357399No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA730.12791757201934534No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC720.12616527651223103No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC710.1244129810051167No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA710.1244129810051167No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.1226606854980024No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.1226606854980024No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT700.1226606854980024No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA690.12090838999088806No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA680.11915609448377375No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.11915609448377375No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.1156515034695451No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA660.1156515034695451No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA660.1156515034695451No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT660.1156515034695451No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC660.1156515034695451No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC650.11389920796243079No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.11214691245531647No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC640.11214691245531647No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA630.11039461694820214No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC630.11039461694820214No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.10689002593397351No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT610.10689002593397351No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.10513773042685919No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT600.10513773042685919No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA600.10513773042685919No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.10338543491974486No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA590.10338543491974486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)