FastQCFastQC Report
Sun 21 May 2023
EGAF00007848337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57068
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA26764.68914277703792No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19293.380178033223523No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13982.449709118945819No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11161.9555617859395809No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA10771.8872222611621223No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10681.8714516015980935No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9801.7172495969720334No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9541.671689913787061No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9501.6646807317586036No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9451.655919254223032No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT8591.5052218406112006No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8271.4491483843835424No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA7831.3720473820705124No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7681.3457629494637975No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7471.3089647438143968No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6791.1898086493306232No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6761.18455176280928No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6481.1354874886100792No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6411.123221420060279No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6201.0864232144108783No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5781.0128268031120768No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA5550.9725240064484475No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5540.9707717109413332No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5510.9655148244199903No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5480.9602579378986472No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5460.9567533468844185No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5420.9497441648559612No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5160.904184481670989No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4940.8656339805144739No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4750.8323403658793018No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4500.788532978201444No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4490.7867806826943297No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4460.7815237961729867No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4410.772762318637415No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4390.7692577276231863No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA4250.7447255905235859No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4240.7429732950164716No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4180.7324595219737857No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4090.7166888624097567No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3990.6991659073386136No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3920.6868998387888133No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3900.6833952477745847No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3810.6676245882105558No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3720.6518539286465269No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3590.6290740870540408No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3450.6045419499544403No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3450.6045419499544403No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3370.5905235858975257No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3270.5730006308263825No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA2960.5186794701058386No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2940.51517487909161No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2920.5116702880773814No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2910.509917992570267No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2880.5046611060489241No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2830.49589962851335245No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.476624377935095No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2650.46435830938529477No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2530.4433307632999229No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2450.42931239924300835No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2270.3977710801149506No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2230.39076189808649336No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2210.3872573070722647No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2080.3644774654797785No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2060.3609728744655499No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.35221139692997827No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1990.34870680591574965No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1950.34169762388729236No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1950.34169762388729236No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.33118385084460644No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.32767925983037777No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1870.32767925983037777No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1860.32592696432326346No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1820.31891778229480616No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.31366089577346323No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1740.30489941823789163No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1720.301394827223663No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1710.29964253171654864No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1610.28211957664540543No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1600.2803672811382911No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.2733580991098339No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1520.2663489170813766No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1520.2663489170813766No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1480.2593397350529193No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1460.2558351440386907No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1380.24181677998177611No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.23655989346043318No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1350.23655989346043318No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1330.2330553024462045No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.2330553024462045No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1310.2295507114319759No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1280.22429382491063293No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1270.2225415294035186No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1200.21027546085371837No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1190.20852316534660406No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC1180.20677086983948972No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.19976168781103248No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.19800939230391812No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.1962570967968038No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1110.1945048012896895No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.1945048012896895No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1100.1927525057825752No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1090.19100021027546085No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC1090.19100021027546085No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.18924791476834654No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1070.18749561926123223No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.18223873273988925No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1020.17873414172566063No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1010.1769818462185463No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA990.17347725520431764No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA980.17172495969720333No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA980.17172495969720333No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT960.16822036868297469No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT950.16646807317586038No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT950.16646807317586038No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT950.16646807317586038No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT930.16296348216163173No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT920.16121118665451742No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.15770659564028877No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.15770659564028877No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC900.15770659564028877No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA890.15595430013317446No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.15420200462606012No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC860.1506974136118315No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC830.14544052709048852No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT810.1419359360762599No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT790.13843134506203125No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.13843134506203125No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.13142216303357399No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC750.13142216303357399No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC740.12966986752645965No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.12791757201934534No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA730.12791757201934534No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.12791757201934534No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA720.12616527651223103No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA710.1244129810051167No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC710.1244129810051167No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.12090838999088806No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC690.12090838999088806No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA680.11915609448377375No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT680.11915609448377375No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.11740379897665941No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC660.1156515034695451No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.1156515034695451No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC660.1156515034695451No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC660.1156515034695451No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA650.11389920796243079No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.11214691245531647No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA620.10864232144108782No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA620.10864232144108782No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.10513773042685919No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.10338543491974486No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT590.10338543491974486No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA590.10338543491974486No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA580.10163313941263055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)