FastQCFastQC Report
Sun 21 May 2023
EGAF00007848338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47438
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21824.599688013828576No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17143.613137147434546No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11972.5232935621231927No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9031.9035372486192506No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8521.7960285003583625No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8461.78338041232767No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7041.4840423289346094No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6801.4334499768118385No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6661.403937771406889No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6481.3659935073148108No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6471.3638854926430288No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6161.2985370378177832No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5951.254268729710359No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5751.2121084362747165No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5691.1994603482440238No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5681.1973523335722416No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5631.186812260213331No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5121.0793035119524432No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5111.0771954972806612No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4931.0392512331885828No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4730.9970909397529407No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4710.9928749104093764No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4640.9781188077069016No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4570.9633627050044268No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4510.9507146169737342No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4390.9254184409123487No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4010.8453138833846284No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3960.8347738100257178No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.8094776339643324No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3830.8073696192925502No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3750.7905055019182934No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3560.7504532231544332No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3530.7441291791390868No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3460.729373076436612No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3420.7209410177494835No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3340.7040769003752266No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3320.6998608710316624No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3220.6787807243138412No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3100.6534845482524558No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3070.6471605042371095No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3040.6408364602217631No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2980.6281883721910704No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2970.6260803575192884No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2890.6092162401450314No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2860.602892196129685No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.5965681521143388No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2790.5881360934272103No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2720.5733799907247354No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2640.5565158733504785No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2570.5417597706480037No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2560.5396517559762216No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2500.527003667945529No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2470.5206796239301825No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2390.5038155065559257No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2310.4869513891816687No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2260.47641131582275814No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2230.4700872718074118No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2170.4574391837767191No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2120.4468991104178085No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.44479109574602643No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2090.4405750664024622No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2090.4405750664024622No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2090.4405750664024622No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2070.43635903705889795No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.42792697837176946No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2030.42792697837176946No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1990.419494919684641No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1960.41317087566929467No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1880.3963067582950377No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1880.3963067582950377No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1830.38576668493612715No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1810.3815506555925629No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1720.36257852354652387No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1690.3562544795311775No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1660.3499304355158312No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.33517433281335635No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1580.3330663181415742No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1580.3330663181415742No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.3225262447826637No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1470.309878156751971No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1340.2824739660188035No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1320.27825793667523924No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1250.2635018339727645No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.25928580462920026No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1230.25928580462920026No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1200.2529617606138539No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.24663771659850753No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.2445297019267254No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1150.24242168725494329No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1150.24242168725494329No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1040.21923352586534No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1020.21501749652177582No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA990.20869345250642946No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT990.20869345250642946No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA940.19815337914751885No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA930.19604536447573673No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA920.19393734980395463No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT910.1918293351321725No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA910.1918293351321725No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA890.1876133057886083No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA890.1876133057886083No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC870.18339727644504406No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA870.18339727644504406No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT850.1791812471014798No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC840.17707323242969772No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC800.16864117374256923No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT780.16442514439900502No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA770.1623171297272229No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.15810110038365865No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT750.15810110038365865No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA730.15388507104009444No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC730.15388507104009444No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.1496690416965302No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA690.14545301235296598No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.14545301235296598No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.14334499768118386No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.14334499768118386No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.14123698300940174No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC670.14123698300940174No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.13912896833761962No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC650.13702095366583752No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT630.13280492432227328No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA630.13280492432227328No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.12858889497870907No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.12858889497870907No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA610.12858889497870907No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA600.12648088030692695No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.12648088030692695No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.1222648509633627No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.1222648509633627No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT580.1222648509633627No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA580.1222648509633627No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.1201568362915806No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.11804882161979847No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG560.11804882161979847No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC560.11804882161979847No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.11594080694801637No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.11594080694801637No Hit
TGTGAGCTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA540.11383279227623425No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA540.11383279227623425No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.11172477760445212No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT530.11172477760445212No Hit
TGTGAGCTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA520.10961676293267No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT520.10961676293267No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA520.10961676293267No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC510.10750874826088791No Hit
TGTGAGCTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA500.10540073358910579No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT500.10540073358910579No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG490.10329271891732367No Hit
TGTGAGCTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA480.10118470424554155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)