FastQCFastQC Report
Sun 21 May 2023
EGAF00007848351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66933
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA32014.782394334633141No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA30524.559783664261277No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9841.4701268432611716No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9791.4626566865372834No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9741.4551865298133955No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9741.4551865298133955No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9701.449210404434285No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9671.4447283103999522No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9461.4133536521596224No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8981.3416401476102968No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8801.3147475834042999No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT8631.2893490505430805No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8501.2699266430609712No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8471.2654445490266386No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8371.2505042355788625No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8361.249010204234085No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8141.2161415146489774No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7811.166838480271316No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7701.1504041354787624No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7651.1429339787548742No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7591.1339697906862087No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6791.0144472831039995No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6761.0099651890696666No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA6610.9875547188980025No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6490.9696263427606711No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA6240.9322755591412308No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG6220.9292874964516756No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5940.8874546187979023No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5900.8814784934187919No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5750.8590680232471276No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5150.7694261425604709No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4960.7410395470096962No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4780.7141469828036993No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4640.6932305439768126No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4570.6827723245633693No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4160.6215170394274873No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4120.6155409140483767No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3760.5617557856363826No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3600.5378512841199408No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3560.5318751587408304No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3530.5273930647064976No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3480.5199229079826095No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3470.5184288766378319No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3440.513946782603499No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3390.506476625879611No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3380.5049825945348333No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3270.48854824974227956No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3240.4840661557079467No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3190.47659599898405874No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3140.4691258422601706No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3130.467631810915393No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3130.467631810915393No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3120.4661377795706154No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2920.4362571526750631No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2900.43326908998550784No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2860.4272929646063975No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2840.4243049019168422No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2820.421316839227287No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2790.41683474519295416No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2710.40488249443473323No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2700.40338846308995563No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2690.401894431745178No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2650.39591830636606756No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2610.3899421809869571No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2500.37350783619440336No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2470.3690257421600705No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2430.3630496167809601No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2430.3630496167809601No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2360.35259139736751677No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2350.35109736602273917No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2330.3481093033331839No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2280.34063914660929584No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2210.33018092719585257No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2200.32868689585107497No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2170.3242048018167421No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC2050.30627642567941077No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2050.30627642567941077No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2050.30627642567941077No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1940.289842080886857No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1860.2778898301286361No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.2763957987838585No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1810.27041967340474804No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1760.26294951668086No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1710.2554793599569719No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1700.2539853286121943No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1690.25249129726741665No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1620.24203307785397335No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1590.23755098381964054No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1570.2345629211300853No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1560.2330688897853077No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1550.23157485844053008No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1550.23157485844053008No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1530.22858679575097485No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1530.22858679575097485No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1520.22709276440619725No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1510.22559873306141962No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1500.224104701716642No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1490.22261067037186438No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.21663454499275392No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1430.21364648230319874No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1400.2091643882688659No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.2091643882688659No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1370.20468229423453305No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.19721213751064498No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1310.19571810616586735No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1270.18974198078675691No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1270.18974198078675691No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.18675391809720168No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.18376585540764645No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1230.18376585540764645No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1210.18077779271809125No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1210.18077779271809125No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1200.17928376137331362No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1200.17928376137331362No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1200.17928376137331362No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1150.17181360464942555No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1140.17031957330464792No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.16733151061509272No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1120.16733151061509272No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.16733151061509272No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1120.16733151061509272No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1070.15986135389120462No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1070.15986135389120462No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1060.15836732254642702No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.15687329120164942No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.15687329120164942No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.15687329120164942No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1010.15089716582253895No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1010.15089716582253895No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1000.14940313447776135No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA980.14641507178820612No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA960.1434270090986509No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT950.14193297775387329No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT950.14193297775387329No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC920.13745088371954042No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA920.13745088371954042No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA900.1344628210299852No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.1344628210299852No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC890.1329687896852076No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT890.1329687896852076No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA880.13147475834043No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA860.12848669565087475No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA850.12699266430609715No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA850.12699266430609715No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC840.12549863296131952No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.12251057027176429No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.12251057027176429No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC800.11952250758220906No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT800.11952250758220906No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC790.11802847623743147No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC770.11504041354787624No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.11354638220309862No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT750.112052350858321No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT750.112052350858321No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC740.11055831951354339No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA740.11055831951354339No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC720.10757025682398816No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG700.10458219413443295No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA690.10308816278965532No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.10308816278965532No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.10308816278965532No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA690.10308816278965532No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT670.10010010010010009No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT670.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)