FastQCFastQC Report
Sun 21 May 2023
EGAF00007848365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49000
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20094.1000000000000005No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13912.8387755102040817No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11712.389795918367347No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10092.059183673469388No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9992.038775510204082No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7991.6306122448979592No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7401.5102040816326532No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7311.4918367346938775No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7261.4816326530612245No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6821.3918367346938776No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6661.3591836734693878No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6461.3183673469387753No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6071.2387755102040816No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6031.2306122448979593No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6001.2244897959183674No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5911.2061224489795919No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5851.193877551020408No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5741.1714285714285713No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5681.1591836734693879No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5641.1510204081632653No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5211.063265306122449No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4880.9959183673469387No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4840.9877551020408163No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4780.9755102040816326No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4410.8999999999999999No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.8551020408163265No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4040.8244897959183672No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4000.8163265306122449No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3900.7959183673469388No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3830.7816326530612245No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3770.7693877551020408No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3750.7653061224489796No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3600.7346938775510203No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3480.710204081632653No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3350.6836734693877551No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.6816326530612246No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3300.673469387755102No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3190.6510204081632653No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3190.6510204081632653No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3070.6265306122448979No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3040.6204081632653061No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3010.6142857142857142No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2990.610204081632653No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2980.6081632653061225No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2960.6040816326530613No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2860.5836734693877551No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2820.5755102040816327No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.5673469387755102No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.5428571428571428No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2580.5265306122448979No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2580.5265306122448979No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2400.4897959183673469No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2380.48571428571428565No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2350.4795918367346939No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2290.4673469387755102No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2230.45510204081632655No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2210.45102040816326533No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2210.45102040816326533No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2130.43469387755102046No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2130.43469387755102046No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2120.4326530612244898No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2090.42653061224489797No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2010.41020408163265304No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.39387755102040817No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1850.3775510204081633No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1760.35918367346938773No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1760.35918367346938773No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1760.35918367346938773No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1730.35306122448979593No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.33877551020408164No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1630.3326530612244898No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1610.32857142857142857No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1540.3142857142857143No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1460.2979591836734694No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.29387755102040813No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.29183673469387755No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA1420.28979591836734697No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1390.2836734693877551No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1340.27346938775510204No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.2714285714285714No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1320.2693877551020408No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1300.2653061224489796No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.25918367346938775No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.2571428571428571No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1240.25306122448979596No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1230.2510204081632653No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.24897959183673468No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1200.24489795918367346No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1200.24489795918367346No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1190.24285714285714283No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1190.24285714285714283No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1170.23877551020408164No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1170.23877551020408164No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1160.23673469387755103No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1160.23673469387755103No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1110.22653061224489796No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1090.22244897959183674No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.22244897959183674No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.2204081632653061No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1080.2204081632653061No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1070.2183673469387755No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.2163265306122449No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.2142857142857143No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1020.20816326530612245No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.2061224489795918No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1000.20408163265306123No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA990.20204081632653062No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT990.20204081632653062No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA970.19795918367346937No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.19795918367346937No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT950.19387755102040816No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT950.19387755102040816No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT940.19183673469387755No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT920.18775510204081633No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.18571428571428572No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA890.1816326530612245No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC880.17959183673469387No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT870.17755102040816326No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT850.17346938775510204No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA840.17142857142857143No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC830.16938775510204082No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA820.1673469387755102No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.16530612244897958No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT800.163265306122449No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT800.163265306122449No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA800.163265306122449No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC780.15918367346938775No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.15510204081632653No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA740.1510204081632653No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA720.14693877551020407No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA720.14693877551020407No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC720.14693877551020407No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT710.14489795918367349No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.14489795918367349No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA700.14285714285714285No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC700.14285714285714285No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC690.14081632653061224No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.13673469387755102No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC670.13673469387755102No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.1346938775510204No Hit
ATACACCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG660.1346938775510204No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC630.12857142857142856No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCAAGCGTTA630.12857142857142856No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.12857142857142856No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCAAGCGTTA610.12448979591836734No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.12448979591836734No Hit
ATACACCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA610.12448979591836734No Hit
ATACACCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA610.12448979591836734No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT600.12244897959183673No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT600.12244897959183673No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT590.12040816326530614No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT590.12040816326530614No Hit
ATACACCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.11836734693877551No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.11632653061224489No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC570.11632653061224489No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.11020408163265305No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC530.10816326530612246No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC530.10816326530612246No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.10816326530612246No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT510.10408163265306122No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.10408163265306122No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC500.10204081632653061No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT500.10204081632653061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)