FastQCFastQC Report
Sun 21 May 2023
EGAF00007848366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62440
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA35245.643818065342729No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21523.44650864830237No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20553.291159513132607No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA15352.4583600256245997No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG13462.1556694426649585No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12672.0291479820627805No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11741.8802049967969252No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9731.5582959641255605No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9371.5006406149903906No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9341.49583600256246No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8571.3725176169122357No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8321.3324791800128122No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8031.2860345932094812No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7941.2716207559256887No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7811.2508007687379885No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7621.2203715566944267No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7521.2043561819346573No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7511.2027546444586803No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7421.188340807174888No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7061.130685458039718No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6851.0970531710442024No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6511.0426008968609866No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6481.0377962844330557No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6471.036194746957079No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6401.0249839846252402No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6331.0137732222934017No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5680.9096732863549006No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5270.8440102498398463No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5220.8360025624599615No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5110.8183856502242152No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5090.8151825752722613No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4920.7879564381806534No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4720.7559256886611146No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4510.722293401665599No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4410.7062780269058296No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4390.7030749519538757No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4340.695067264573991No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.6902626521460602No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4060.6502242152466368No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4050.6486226777706599No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT4010.6422165278667521No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3950.6326073030108905No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3890.6229980781550288No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3830.6133888532991673No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3690.5909673286354901No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3680.5893657911595132No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3550.568545803971813No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3400.5445227418321589No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3330.5333119795003203No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3200.5124919923126201No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3160.5060858424087123No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3080.49327354260089684No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2920.4676489429852658No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2810.45003203074951953No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2790.44682895579756565No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2760.44202434336963486No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2750.4404228058936579No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2740.438821268417681No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2570.41159513132607306No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2570.41159513132607306No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2550.40839205637411913No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2440.39077514413837283No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2440.39077514413837283No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2360.3779628443305573No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.36194746957078794No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2220.35554131966688024No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2190.3507367072389494No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2140.3427290198590647No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2130.34112748238308777No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2050.32831518257527226No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1860.29788597053171045No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1770.28347213324791803No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1760.28187059577194107No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1750.2802690582959641No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1700.27226137091607944No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1670.26745675848814865No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.26105060858424084No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1610.257847533632287No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1590.25464445868033314No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1580.2530429212043562No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1560.24983984625240233No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1550.24823830877642536No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1550.24823830877642536No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.23862908392056373No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1480.2370275464445868No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1460.2338244714926329No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1410.22581678411274825No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1350.21620755925688662No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1330.2130044843049327No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1310.20980140935297886No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1240.19859064702114027No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1230.19698910954516335No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1230.19698910954516335No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1200.19218449711723257No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.18898142216527866No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.18577834721332478No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1160.18577834721332478No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1150.18417680973734785No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1140.18257527226137094No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1120.17937219730941703No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1110.17777065983344012No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1110.17777065983344012No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.17616912235746315No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1080.1729660474055093No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1050.1681614349775785No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1010.1617552850736707No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1000.1601537475976938No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.1601537475976938No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA990.15855221012171686No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC980.1569506726457399No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC970.15534913516976298No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT960.15374759769378604No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC940.15054452274183217No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC940.15054452274183217No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC940.15054452274183217No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT930.14894298526585523No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.14573991031390135No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC880.14093529788597053No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA870.1393337604099936No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA860.13773222293401666No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA850.13613068545803972No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.13292761050608584No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA830.13292761050608584No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT830.13292761050608584No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC830.13292761050608584No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.13292761050608584No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA830.13292761050608584No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA810.12972453555413196No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT780.12491992312620116No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA780.12491992312620116No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA780.12491992312620116No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.12331838565022421No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.12171684817424727No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.12011531069827035No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.11691223574631646No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC730.11691223574631646No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA720.11531069827033953No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC720.11531069827033953No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT720.11531069827033953No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT720.11531069827033953No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC710.11370916079436258No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA690.11050608584240872No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT680.10890454836643176No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA670.10730301089045484No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC670.10730301089045484No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT650.10409993593850095No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC650.10409993593850095No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA650.10409993593850095No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC640.10249839846252402No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT640.10249839846252402No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT630.10089686098654709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)