FastQCFastQC Report
Sun 21 May 2023
EGAF00007848372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50672
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA29755.871092516577202No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17693.491079886327755No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12072.381986106725608No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10472.066229870539943No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9141.8037574992106093No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8151.6083833280707294No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7691.5176034101673508No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7561.4919482159772655No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7021.3853804862646037No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6971.3755131038838015No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6541.2906536154089043No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6121.2077676034101674No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5981.1801389327439218No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5951.1742185033154404No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5921.1682980738869593No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5541.0933059677928638No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5501.0854120618882224No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5461.0775181559835807No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5341.053836438269656No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5301.0459425323650142No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5171.020287338174929No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4880.9630565203662773No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4770.9413482791285128No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4660.9196400378907484No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4510.8900378907483422No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4440.8762235554152195No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4380.8643826965582571No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4350.8584622671297759No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.8505683612251341No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4140.8170192611304073No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3900.7696558257025576No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3720.7341332491316703No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3710.7321597726555099No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3620.7143984843700663No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3470.6847963372276602No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3410.6729554783706978No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3320.6551941900852543No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3300.6512472371329334No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3300.6512472371329334No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3230.6374329017998105No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3020.595989895800442No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2970.5861225134196401No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2890.5703347016103568No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2850.5624407957057151No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2790.5505999368487527No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2780.5486264603725923No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2730.5387590779917903No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2680.5288916956109884No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2640.5209977897063467No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2640.5209977897063467No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2630.5190243132301863No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2620.5170508367540259No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.5012630249447427No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2450.483501736659299No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2380.46968740132617615No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2330.4598200189453742No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2330.4598200189453742No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2290.45192611304073255No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2240.4420587306599305No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2180.4302178718029681No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2110.4164035364698453No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2110.4164035364698453No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2100.4144300599936849No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2100.4144300599936849No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2090.4124565835175245No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2060.4065361540890432No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2050.4045626776128829No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2020.3986422481844017No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1970.3887748658035996No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1940.3828544363751184No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1820.35917271866119355No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.35325228923271235No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1710.3374644774234291No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1680.33154404799494785No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1630.3216766656141459No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.3216766656141459No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1590.3137827597095043No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1590.3137827597095043No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.30786233028102306No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1460.28812756551941904No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.2841806125670982No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1430.28220713609093784No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1430.28220713609093784No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1410.278260183138617No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1350.26641932428165455No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1340.2644458478054942No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.2447110830438901No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.24076413009156933No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.24076413009156933No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1210.2387906536154089No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1200.23681717713924852No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1130.22300284180612567No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1130.22300284180612567No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1100.21708241237764445No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1090.21510893590148406No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.19932112409220085No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT980.1934006946637196No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC980.1934006946637196No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA960.1894537417113988No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA960.1894537417113988No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT950.1874802652352384No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.17958635933059677No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT900.17761288285443638No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA900.17761288285443638No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA890.17563940637827596No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA880.17366592990211557No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.17366592990211557No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.17366592990211557No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT870.17169245342595515No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG870.17169245342595515No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.17169245342595515No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC870.17169245342595515No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT860.16971897694979476No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT820.16182507104515315No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA790.15590464161667192No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA790.15590464161667192No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC760.14998421218819072No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC760.14998421218819072No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT750.14801073571203033No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG750.14801073571203033No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA740.1460372592358699No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.1460372592358699No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA730.14406378275970952No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA720.1420903062835491No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA710.14011682980738868No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC710.14011682980738868No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT710.14011682980738868No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA700.1381433533312283No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.13616987685506787No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.1322229239027471No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.1322229239027471No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC650.12827597095042628No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA640.12630249447426586No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.12235554152194505No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC620.12235554152194505No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.12235554152194505No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.12038206504578466No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA600.11840858856962426No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.11643511209346386No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.11446163561730344No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC560.11051468266498263No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA550.10854120618882222No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.10854120618882222No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT540.10656772971266183No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.10459425323650143No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT530.10459425323650143No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC530.10459425323650143No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC520.10262077676034102No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC510.10064730028418062No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA510.10064730028418062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)