FastQCFastQC Report
Sun 21 May 2023
EGAF00007848373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50672
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA27675.460609409535838No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15873.1319071676665615No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11552.279365329965267No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9361.8471739816861381No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8111.600489422166088No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7911.5610198926428798No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7821.5432586043574361No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7421.4643195453110198No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7211.4228765393116514No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7151.411035680454689No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7081.397221345121566No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7021.3853804862646037No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6911.3636722450268393No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6821.3459109567413956No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6561.2946005683612252No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6241.2314493211240922No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6151.2136880328386486No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5711.1268550678875908No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5191.0242342911272497No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5151.016340385222608No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5121.010419955794127No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5040.9946321439848437No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4700.9275339437953899No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4500.8880644142721819No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4440.8762235554152195No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4380.8643826965582571No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4250.8387275023681717No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4230.8347805494158509No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4130.8150457846542469No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3900.7696558257025576No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3780.7459741079886327No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3740.7380802020839912No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3590.7084780549415851No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3570.7045311019892643No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3550.7005841490369434No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3530.6966371960846227No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3490.688743290179981No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3470.6847963372276602No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3450.6808493842753395No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3270.6453268077044522No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.619671613514367No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2990.5900694663719609No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2990.5900694663719609No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2930.5782286075149985No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2850.5624407957057151No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2850.5624407957057151No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2780.5486264603725923No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2760.5446795074202715No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2730.5387590779917903No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2610.5150773602778655No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2450.483501736659299No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2430.47955478370697824No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2400.47363435427849704No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2370.46771392485001584No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2360.4657404483738554No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2350.46376697189769495No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2300.453899589516893No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.44403220713609093No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2210.4361383012314493No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.43219134827912853No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2180.4302178718029681No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2050.4045626776128829No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.3966687717082413No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2000.3946952952320808No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1960.3868013893274392No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1950.3848279128512788No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1940.3828544363751184No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1920.3789074834227976No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1890.37298705399431636No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1870.3690401010419956No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1860.36706662456583516No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1740.3433849068519103No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1700.3354910009472687No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1700.3354910009472687No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.3295705715187875No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1600.3157562361856647No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1590.3137827597095043No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1580.31180928323334384No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.30786233028102306No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.29404799494790024No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1410.278260183138617No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.27431323018629616No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1370.2703662772339754No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1310.2585254183770129No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1300.25655194190085256No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1270.25063151247237136No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.24668455952005053No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1240.2447110830438901No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1200.23681717713924852No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1190.23484370066308807No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1170.23089674771076726No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.23089674771076726No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1170.23089674771076726No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1160.22892327123460687No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1140.22497631828228606No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1120.22102936532996525No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1050.20721502999684244No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.20524155352068205No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.20524155352068205No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1040.20524155352068205No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1040.20524155352068205No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1030.2032680770445216No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.2032680770445216No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA980.1934006946637196No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA960.1894537417113988No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA920.18155983580675716No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG920.18155983580675716No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT910.17958635933059677No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC900.17761288285443638No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA890.17563940637827596No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA880.17366592990211557No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.17169245342595515No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA850.16774550047363435No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA830.16379854752131356No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.16379854752131356No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.15985159456899273No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA800.15787811809283234No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT780.15393116514051153No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT770.1519576886643511No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.14801073571203033No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC740.1460372592358699No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.14406378275970952No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT710.14011682980738868No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.13419640037890748No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.13024944742658667No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG650.12827597095042628No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC650.12827597095042628No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.12827597095042628No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.12630249447426586No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT630.12432901799810546No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA620.12235554152194505No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.12038206504578466No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTA610.12038206504578466No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA600.11840858856962426No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA600.11840858856962426No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC600.11840858856962426No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.11643511209346386No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.11446163561730344No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA560.11051468266498263No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA560.11051468266498263No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.11051468266498263No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT550.10854120618882222No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC550.10854120618882222No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC550.10854120618882222No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC550.10854120618882222No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA540.10656772971266183No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC540.10656772971266183No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT540.10656772971266183No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.10656772971266183No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.10459425323650143No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC530.10459425323650143No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA520.10262077676034102No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT520.10262077676034102No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.10064730028418062No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC510.10064730028418062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)