FastQCFastQC Report
Sun 21 May 2023
EGAF00007848376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43630
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12442.851249140499656No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11272.5830850332340134No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11032.5280770112308044No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10582.4249369699747882No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9472.1705248682099474No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9112.088012835205134No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6621.5173046069218428No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6111.4004125601650241No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6001.37520055008022No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5821.3339445335778135No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5731.3133165253266101No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5681.3018565207426083No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5361.2285124914049965No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5251.2033004813201926No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5061.1597524639009855No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4911.1253724501489801No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4701.0772404308961723No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4651.0657804263121704No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4631.0611964244785699No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4631.0611964244785699No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4320.9901443960577584No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4220.9672243868897548No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4090.93742837497135No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3800.8709603483841394No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3710.850332340132936No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3680.843456337382535No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3640.8342883337153335No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3640.8342883337153335No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3590.8228283291313316No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3560.8159523263809306No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3520.8067843227137291No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3460.7930323172129269No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3290.7540683016273206No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2980.6830162732065093No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2950.6761402704561081No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.6532202612881045No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2780.6371762548705019No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2770.6348842539537016No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2680.6142562457024983No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2610.5982122392848956No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2600.5959202383680954No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2550.5844602337840935No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2510.575292230116892No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2450.5615402246160899No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2420.5546642218656888No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2410.5523722209488884No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2310.5294522117808848No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.5294522117808848No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2140.49048819619527845No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2100.481320192528077No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2050.4698601879440752No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1990.45610818244327295No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1980.45381618152647263No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1970.4515241806096722No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.4515241806096722No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.4354801741920697No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1860.42631217052486825No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1790.41026816410726563No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.4079761631904653No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1770.4056841622736649No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1710.3919321567728627No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1690.387348154939262No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1690.387348154939262No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1680.3850561540224616No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1680.3850561540224616No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1570.35984414393765757No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.35755214302085725No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.35755214302085725No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1530.3506761402704561No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1500.343800137520055No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1480.33921613568645426No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1420.32546413018565207No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.3162961265184506No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1360.3117121246848499No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1250.2865001146000459No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1250.2865001146000459No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.2819161127664451No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1220.27962411184964475No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.27962411184964475No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1210.2773321109328444No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1200.275040110016044No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1200.275040110016044No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1180.2704561081824433No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.2681641072656429No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1160.2658721063488425No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1110.2544121017648407No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.2521201008480403No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1100.2521201008480403No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1100.2521201008480403No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1070.24524409809763922No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1020.2337840935136374No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1010.23149209259683703No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1000.22920009168003666No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC990.22690809076323631No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC980.22461608984643594No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA970.22232408892963557No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC970.22232408892963557No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA950.21774008709603485No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA900.20628008251203303No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT890.20398808159523266No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA880.20169608067843228No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG870.19940407976163194No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.19711207884483156No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG860.19711207884483156No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA850.19482007792803116No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA850.19482007792803116No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA840.1925280770112308No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA830.19023607609443044No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.18565207426082972No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT810.18565207426082972No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA790.18106807242722897No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA780.17877607151042862No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.17648407059362825No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT770.17648407059362825No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.1719000687600275No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT740.16960806784322713No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA740.16960806784322713No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT720.1650240660096264No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.16273206509282603No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.16273206509282603No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA710.16273206509282603No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA700.16044006417602566No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA660.1512720605088242No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA650.14898005959202384No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC630.1443960577584231No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.1443960577584231No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA620.14210405684162275No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.14210405684162275No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.137520055008022No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA600.137520055008022No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC590.13522805409122166No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.13522805409122166No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC570.1306440522576209No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.1306440522576209No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC570.1306440522576209No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.12835205134082053No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.12835205134082053No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC540.1237680495072198No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC540.1237680495072198No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT530.12147604859041944No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA530.12147604859041944No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC530.12147604859041944No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.11918404767361906No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC520.11918404767361906No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC520.11918404767361906No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA510.1168920467568187No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA510.1168920467568187No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA500.11460004584001833No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.11460004584001833No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA500.11460004584001833No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.11460004584001833No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG490.11230804492321797No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.11001604400641761No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.10772404308961724No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT470.10772404308961724No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.10772404308961724No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC470.10772404308961724No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT470.10772404308961724No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA470.10772404308961724No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT460.10543204217281688No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA460.10543204217281688No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG460.10543204217281688No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT460.10543204217281688No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT460.10543204217281688No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA450.10314004125601652No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA450.10314004125601652No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC440.10084804033921614No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT440.10084804033921614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)