FastQCFastQC Report
Sun 21 May 2023
EGAF00007848377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43630
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12302.8191611276644513No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG10952.509741003896402No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9572.1934448773779507No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9362.145312858125143No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8261.893192757277103No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7481.7144166857666743No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6971.5975246390098556No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6801.5585606234242493No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6691.5333486133394454No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6431.4737565895026359No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6301.443960577584231No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6271.43708457483383No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5631.2903965161586064No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5491.2583085033234014No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5211.1941324776529911No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5201.1918404767361908No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4781.0955764382305753No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4721.0818244327297732No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4671.0703644281457714No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4641.06348842539537No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4591.0520284208113684No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4541.0405684162273665No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4481.0268164107265643No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4451.0199404079761631No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4200.962640385056154No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3970.9099243639697456No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.7792803117121246No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3360.7701123080449233No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3220.7380242952097181No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3200.7334402933761174No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3110.712812285124914No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.705936282374513No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3080.705936282374513No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2960.6784322713729085No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.6761402704561081No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.6738482695393078No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2800.6417602567041026No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2720.6234242493696998No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2650.6073802429520971No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2650.6073802429520971No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2640.6050882420352968No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2630.6027962411184964No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2470.5661242264496905No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2450.5615402246160899No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2390.5477882191152876No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.5157002062800825No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2250.5157002062800825No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2250.5157002062800825No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.5088242035296814No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2170.4973641989456796No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2110.4836121934448774No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2080.47673619069447626No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.46298418519367407No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1900.4354801741920697No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1890.43318817327526926No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.4148521659408664No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1800.41256016502406606No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.4056841622736649No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1740.39880815952326387No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.39651615860646344No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1730.39651615860646344No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1720.3942241576896631No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1690.387348154939262No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1650.3781801512720605No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1600.3667201466880587No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1580.36213614485445794No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1570.35984414393765757No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1550.3552601421040569No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1490.34150813660325463No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1440.3300481320192528No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.3208801283520513No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1390.31858812743525095No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1350.3094201237680495No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1350.3094201237680495No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1320.3025441210176484No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1320.3025441210176484No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1300.2979601191840477No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.2887921155168462No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1250.2865001146000459No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1250.2865001146000459No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1240.2842081136832455No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1210.2773321109328444No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1210.2773321109328444No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.275040110016044No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1190.2727481090992437No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.2681641072656429No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1170.2681641072656429No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1150.26358010543204213No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1110.2544121017648407No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.2475360990144396No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.2406600962640385No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1030.23607609443043776No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1010.23149209259683703No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA980.22461608984643594No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC940.21544808617923447No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA920.21086408434563375No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC920.21086408434563375No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG920.21086408434563375No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC920.21086408434563375No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA890.20398808159523266No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA870.19940407976163194No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA860.19711207884483156No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA850.19482007792803116No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA840.1925280770112308No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT820.18794407517763007No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT810.18565207426082972No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA800.18336007334402935No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.18336007334402935No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA800.18336007334402935No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA790.18106807242722897No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT770.17648407059362825No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA770.17648407059362825No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.17419206967682788No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.1719000687600275No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC750.1719000687600275No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT750.1719000687600275No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT740.16960806784322713No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG700.16044006417602566No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT690.1581480632592253No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.15585606234242494No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA680.15585606234242494No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.15356406142562457No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT670.15356406142562457No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC660.1512720605088242No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA640.14668805867522347No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA640.14668805867522347No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA620.14210405684162275No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.13981205592482238No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT610.13981205592482238No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA600.137520055008022No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA600.137520055008022No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA590.13522805409122166No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.13522805409122166No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.13522805409122166No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.13293605317442125No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC580.13293605317442125No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT580.13293605317442125No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC570.1306440522576209No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG570.1306440522576209No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA560.12835205134082053No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.12606005042402016No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.12606005042402016No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC540.1237680495072198No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA540.1237680495072198No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC540.1237680495072198No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.1237680495072198No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.12147604859041944No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.12147604859041944No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC530.12147604859041944No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA520.11918404767361906No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT520.11918404767361906No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC510.1168920467568187No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT500.11460004584001833No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.11460004584001833No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC500.11460004584001833No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA460.10543204217281688No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA460.10543204217281688No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA460.10543204217281688No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.10543204217281688No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC450.10314004125601652No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC450.10314004125601652No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT450.10314004125601652No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG450.10314004125601652No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA450.10314004125601652No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA440.10084804033921614No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA440.10084804033921614No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG440.10084804033921614No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT440.10084804033921614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)