FastQCFastQC Report
Sun 21 May 2023
EGAF00007848386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47390
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG21484.532601814728846No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG18373.876345220510656No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13882.928887951044524No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9461.9962017303228528No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9071.9139058873179995No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8451.783076598438489No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8041.6965604557923613No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7891.6649082084828022No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7611.6058240135049588No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6301.329394387001477No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6171.3019624393331926No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5951.255539143279173No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA5441.1479215024266722No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5301.1183794049377507No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5291.1162692551171134No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5211.0993880565520153No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA5101.0761764085250054No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4921.0381937117535345No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4730.9981008651614264No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4570.9643384680312302No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4390.9263557712597594No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4220.890483224308926No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4100.8651614264612787No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4000.8440599282549061No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3790.7997467820215235No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3770.795526482380249No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3730.7870858830976999No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3700.7807554336357881No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3690.7786452838151509No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3690.7786452838151509No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3610.7617640852500528No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3430.723781388478582No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3420.7216712386579447No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3320.700569740451572No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3260.6879088415277484No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3190.6731377927832876No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3000.6330449461911796No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3000.6330449461911796No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2890.6098332981641696No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2890.6098332981641696No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2870.6056129985228951No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2730.5760709010339734No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.5718506013926988No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2700.5697404515720617No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.5697404515720617No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2690.5676303017514244No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.5612998522895126No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2640.557079552648238No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2560.5401983540831399No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.5359780544418654No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2510.5296476049799536No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2470.5212070056974045No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2420.5106562565942182No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2360.49799535767039466No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2180.4600126608989238No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2010.4241401139480903No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.42202996412745303No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2000.42202996412745303No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.413589364844904No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1910.4030386157417177No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.4030386157417177No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.3924878666385313No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.3924878666385313No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1780.3756066680734332No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.36716606879088415No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1630.3439544207638742No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1570.3312935218400506No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1570.3312935218400506No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1560.32918337201941333No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.3270732221987761No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1510.31863262291622707No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1500.3165224730955898No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.31441232327495255No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1480.31230217345431527No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1470.310192023633678No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1460.30808187381304075No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1420.29964127453049166No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1390.29331082506857986No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1370.2890905254273053No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1360.28698037560666806No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1340.28276007596539354No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1340.28276007596539354No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1260.2658788774002954No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1230.2595484279383836No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1220.25743827811774633No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1220.25743827811774633No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1200.2532179784764718No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT1180.24899767883519733No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1150.24266722937328547No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT1120.2363367799113737No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.23422663009073647No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1110.23422663009073647No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT1100.23211648027009918No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1100.23211648027009918No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1080.22789618062882466No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1060.2236758809875501No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1020.21523528170500106No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.2131251318843638No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA970.2046845326018147No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT950.2004642329605402No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA910.19202363367799113No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA890.1878033340367166No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT880.18569318421607933No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC850.17936273475416756No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC850.17936273475416756No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA840.1772525849335303No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT830.175142435112893No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT810.1709221354716185No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA770.1624815361890694No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT760.16037138636843218No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA750.1582612365477949No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT730.15404093690652038No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC730.15404093690652038No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT720.1519307870858831No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCT720.1519307870858831No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC690.1456003376239713No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC680.14349018780333403No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCT670.14138003798269677No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC650.13715973834142225No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT650.13715973834142225No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT630.1329394387001477No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC620.13082928887951045No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC620.13082928887951045No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA610.12871913905887317No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT610.12871913905887317No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA610.12871913905887317No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT600.1266089892382359No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT600.1266089892382359No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.1266089892382359No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT580.12238868959696138No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA580.12238868959696138No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCT580.12238868959696138No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT570.12027853977632412No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA570.12027853977632412No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCT550.11605824013504959No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT540.11394809031441233No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC540.11394809031441233No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC530.11183794049377505No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT520.10972779067313779No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10972779067313779No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10972779067313779No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACT520.10972779067313779No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA510.10761764085250053No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC510.10761764085250053No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT500.10550749103186326No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCT490.103397341211226No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC480.10128719139058874No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA480.10128719139058874No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT480.10128719139058874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)