FastQCFastQC Report
Sun 21 May 2023
EGAF00007848387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47390
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG20294.281493986073011No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG16813.5471618484912426No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG12622.6630090736442287No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9471.9983118801434903No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9221.9455581346275583No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9111.9223464866005484No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9071.9139058873179995No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9021.9033551382148133No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8611.8168389955686852No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6531.3779278328761342No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6391.3483857353872124No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5851.2344376450728003No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA5831.2302173454315257No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5741.2112259970457901No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5511.162692551171133No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA5191.0951677569107408No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5191.0951677569107408No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5131.082506857986917No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5121.0803967081662798No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4660.9833298164169656No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4340.9158050221565732No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4250.8968136737708376No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4010.8461700780755433No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4000.8440599282549061No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3880.8187381304072588No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3760.7934163325596117No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3720.7849757332770626No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3470.732221987761131No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3410.7195610888373074No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3370.7111204895547584No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3360.7090103397341212No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3290.6942392909896603No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3280.692129141169023No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3240.683688541886474No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3150.6646971935007385No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3130.660476893859464No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3120.6583667440388267No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3100.6541464443975522No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3100.6541464443975522No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA3090.6520362945769149No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3080.6499261447562776No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2980.6288246465499051No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2890.6098332981641696No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2890.6098332981641696No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2880.6077231483435324No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2810.5929520995990715No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2660.5612998522895126No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2480.5233171555180418No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2390.5043258071323063No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.5022156573116691No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2320.4895547583878455No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2310.4874446085672082No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2280.4811141591052965No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2250.4747837096433847No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2130.44946191179573747No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2070.4368010128719139No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.4262502637687276No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2010.4241401139480903No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1980.41780966448617857No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1920.40514876556235496No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.4009284659210804No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1870.3945980164591686No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1830.38615741717661956No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1780.3756066680734332No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1730.36505591897024686No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.36294576914960963No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1720.36294576914960963No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.3587254695083351No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1600.3376239713019624No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1570.3312935218400506No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.3228529225575016No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1530.3228529225575016No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.31863262291622707No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1480.31230217345431527No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1450.30597172399240347No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1450.30597172399240347No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.3038615741717662No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1400.29542097488921715No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1370.2890905254273053No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1320.278539776324119No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1300.2743194766828445No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1270.2679890272209327No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1250.26376872757965814No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1230.2595484279383836No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1230.2595484279383836No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1210.2553281282971091No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT1190.25110782865583453No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1170.24688752901456004No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1150.24266722937328547No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT1120.2363367799113737No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1110.23422663009073647No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.23211648027009918No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1090.2300063304494619No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1090.2300063304494619No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT1080.22789618062882466No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT1080.22789618062882466No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1080.22789618062882466No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1060.2236758809875501No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1060.2236758809875501No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1020.21523528170500106No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA960.20257438278117748No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA930.19624393331926565No Hit
AACTCTTACGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA900.18991348385735388No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA890.1878033340367166No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.18569318421607933No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC860.18147288457480482No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT850.17936273475416756No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT750.1582612365477949No Hit
AACTCTTACGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA680.14349018780333403No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT680.14349018780333403No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT670.14138003798269677No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT670.14138003798269677No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT670.14138003798269677No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT650.13715973834142225No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC640.13504958852078497No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT630.1329394387001477No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC630.1329394387001477No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA620.13082928887951045No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA620.13082928887951045No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA610.12871913905887317No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT600.1266089892382359No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT590.12449883941759866No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC580.12238868959696138No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCT580.12238868959696138No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA580.12238868959696138No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.12027853977632412No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCT570.12027853977632412No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT570.12027853977632412No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC570.12027853977632412No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT570.12027853977632412No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.11816838995568685No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA560.11816838995568685No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC560.11816838995568685No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC550.11605824013504959No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT540.11394809031441233No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCT540.11394809031441233No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT530.11183794049377505No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.10972779067313779No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC520.10972779067313779No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC520.10972779067313779No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCT510.10761764085250053No Hit
AACTCTTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA510.10761764085250053No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA500.10550749103186326No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC500.10550749103186326No Hit
AACTCTTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.10550749103186326No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT500.10550749103186326No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.103397341211226No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.10128719139058874No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCT480.10128719139058874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)