FastQCFastQC Report
Sun 21 May 2023
EGAF00007848391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73482
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21292.897308184317248No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20202.748972537492175No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14201.9324460412073707No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13721.8671239215045863No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG13031.7732233744318335No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12541.7065403772352414No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10971.4928826107073843No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT10381.4125908385727117No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10231.3921776761655915No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9821.3363816989194632No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9771.3295773114504232No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9421.281946599167143No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9351.2724204567104869No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT9281.262894314253831No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9271.2615334367600228No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9211.2533681717971747No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA9131.2424811518467107No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9031.2288723769086307No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA9021.2275114994148228No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8691.1826025421191584No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8601.1703546446748865No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8111.1036716474782942No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7871.0710105876269018No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7851.0682888326392859No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7811.0628453226640537No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7140.9716665305789173No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT7040.9580577556408373No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6810.9267575732832531No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6590.8968182684194769No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6450.8777659835061649No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6370.8668789635557008No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6190.8423831686671567No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6040.8219700062600364No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5900.8029177213467245No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5890.8015568438529164No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5880.8001959663591084No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5870.7988350888653004No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5770.7852263139272203No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5410.736234724150132No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5400.734873846656324No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT5140.6994910318173158No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4960.6749952369287717No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4830.6573038295092676No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4580.6232818921640674No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4470.6083122397321793No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4450.6055904847445632No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4390.5974252197817153No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4360.5933425873002912No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.570207669905555No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3990.542990120029395No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3970.5402683650417789No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3930.5348248550665469No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3890.5293813450913148No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3740.5089681826841947No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3730.5076073051903868No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3700.5035246727089627No Hit
TGCACGCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3640.49535940774611475No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3630.49399853025230667No Hit
TGCACGCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3600.4899158977708827No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3590.4885550202770746No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3510.4776680003266106No Hit
TGCACGCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3240.44092430799379434No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3030.41234588062382627No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3020.4109850031300182No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.374241310797202No Hit
TGCACGCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2720.37015867831577803No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2680.36471516834054596No Hit
TGCACGCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2660.36199341335292995No Hit
TGCACGCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2500.34021937345200187No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2500.34021937345200187No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2420.3293323535015378No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2420.3293323535015378No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2290.3116409460820337No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2260.3075583136006097No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2150.2925886611687216No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA2120.2885060286872976No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2110.28714515119348955No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA2010.2735363762554095No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1990.27081462126779343No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.2680928662801775No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1900.2585667238235214No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1880.25584496883590535No Hit
TGCACGCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.2544840913420974No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1850.25176233635448136No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1830.24904058136686535No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1830.24904058136686535No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1760.23951443891020932No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1680.22862741895974523No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1680.22862741895974523No Hit
TGCACGCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.22590566397212922No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1640.2231839089845132No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1630.2218230314907052No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1630.2218230314907052No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1590.21637952151547318No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1590.21637952151547318No Hit
TGCACGCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.21365776652785715No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1550.21093601154024114No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1520.20685337905881712No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1500.2041316240712011No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC1470.2000489915897771No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC1470.2000489915897771No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1460.19868811409596907No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1440.19596635910835306No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1430.19460548161454505No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1360.18507933915788902No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1350.183718461664081No Hit
TGCACGCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.18099670667646497No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.18099670667646497No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1320.17963582918265697No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1310.17827495168884896No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCC1300.17691407419504096No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1280.17419231920742495No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC1250.17010968672600094No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1220.1660270542445769No Hit
TGCACGCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1210.1646661767507689No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1210.1646661767507689No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1190.16194442176315288No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCC1180.16058354426934487No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1180.16058354426934487No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1160.15786178928172886No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.15514003429411286No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1120.15241827930649682No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1120.15241827930649682No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCC1110.1510574018126888No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1100.1496965243188808No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1090.1483356468250728No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG1080.1469747693312648No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCC1070.1456138918374568No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1070.1456138918374568No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1060.1442530143436488No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1050.14289213684984076No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1030.14017038186222477No Hit
TGCACGCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1030.14017038186222477No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1000.13608774938080073No Hit
TGCACGCTTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT930.12656160692414467No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA930.12656160692414467No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT920.1252007294303367No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT910.12383985193652869No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC910.12383985193652869No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCC900.12247897444272067No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC890.12111809694891267No Hit
TGCACGCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.11975721945510466No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.11975721945510466No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT850.11567458697368063No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA840.11431370947987261No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC840.11431370947987261No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA830.11295283198606461No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC820.1115919544922566No Hit
TGCACGCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1115919544922566No Hit
TGCACGCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT820.1115919544922566No Hit
TGCACGCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.10887019950464061No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA800.10887019950464061No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT800.10887019950464061No Hit
TGCACGCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC790.10750932201083259No Hit
TGCACGCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT790.10750932201083259No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA780.10614844451702458No Hit
TGCACGCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCC780.10614844451702458No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA780.10614844451702458No Hit
TGCACGCTTCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT760.10342668952940856No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA760.10342668952940856No Hit
TGCACGCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.10206581203560056No Hit
TGCACGCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT740.10070493454179255No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA740.10070493454179255No Hit
TGCACGCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.10070493454179255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)