FastQCFastQC Report
Sun 21 May 2023
EGAF00007848418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47687
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTA37867.939270660767085No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTA34427.217900056619205No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTA21654.540021389477216No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTA21124.4288799882567575No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT15623.2755258246482266No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14393.0175938935139555No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14292.996623817811982No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT14122.9609746891186277No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10082.113783630758907No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT9632.019418290100027No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7811.6377629123241133No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT7641.602113783630759No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7631.6000167760605617No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7441.5601736322268123No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6601.3840249963302367No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5451.142869125757544No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5331.117705034915176No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5021.0526978002390588No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4971.0422127623880724No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4580.9604294671503764No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4460.9352653763080084No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4420.9268773460272192No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4350.9121982930358378No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4190.8786461719126806No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3790.7947658691047875No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3660.7675047706922222No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3330.6983035208757103No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3290.6899154905949211No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3280.6878184830247237No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3270.6857214754545264No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3210.6731394300333424No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3090.6479753391909745No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3030.6353932937697905No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2780.5829681045148573No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2750.5766770818042652No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2370.4969907941367669No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2320.4865057562857802No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2290.4802147335751882No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2200.46134166544341226No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2170.45505064273282025No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2160.452953635162623No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2130.4466626124520309No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2040.42778954432025496No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1980.415207498899071No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1920.40262545347788703No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.39633443076729513No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1830.3837523853461111No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1800.3774613626355191No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.35858829450374313No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1690.3543942793633485No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1620.3397152263719672No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1600.3355212112315725No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1480.3103571203892046No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1450.3040660976786126No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1430.29987208253821795No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1340.28099901440644204No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1280.2684169689852581No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.2663199614150607No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1250.262125946274666No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1250.262125946274666No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1220.25583492356407406No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1220.25583492356407406No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1210.25373791599387674No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1200.2516409084236794No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1180.24744689328328476No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1110.23276784029190345No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1100.23067083272170613No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1040.21808878730052214No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.20550674187933818No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA980.20550674187933818No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT930.19502170402835153No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC920.19292469645815422No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA920.19292469645815422No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC910.1908276888879569No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC900.18873068131775955No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT890.18663367374756223No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA870.18243965860716757No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC860.18034265103697023No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA850.1782456434667729No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC850.1782456434667729No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT840.1761486358965756No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG840.1761486358965756No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA840.1761486358965756No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA810.1698576131859836No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT780.16356659047539163No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA770.1614695829051943No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT710.14888753748401032No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA670.14049950720322102No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC650.13630549206282636No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA640.13420848449262904No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT640.13420848449262904No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA620.13001446935223435No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA620.13001446935223435No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA600.1258204542118397No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT590.12372344664164238No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA590.12372344664164238No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC580.12162643907144505No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA570.11952943150124772No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC570.11952943150124772No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA570.11952943150124772No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC560.1174324239310504No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.11533541636085307No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.11533541636085307No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC540.11323840879065575No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT540.11323840879065575No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA530.11114140122045842No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA520.10904439365026107No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC520.10904439365026107No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.10904439365026107No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA510.10694738608006374No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.10485037850986642No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC490.10275337093966909No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC490.10275337093966909No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA490.10275337093966909No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC480.10065636336947176No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA480.10065636336947176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)