FastQCFastQC Report
Sun 21 May 2023
EGAF00007848427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66174
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA23723.584489376492278No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA20063.0314020612325083No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG17232.6037416507994076No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG14552.198748753286789No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG14062.1247015444132136No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12641.9101157554326473No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11211.6940187989240487No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT10961.6562396107232449No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT10261.5504578837609937No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10181.5383685435367365No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9551.4431649892707106No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9411.4220086438782604No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9191.3887629582615528No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9031.3645842778130384No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8741.320760419500106No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8571.295070571523559No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8351.2618248859068517No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7851.1862665095052438No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7691.1620878290567291No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7601.1484873213044398No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7531.1379091486082147No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7401.1182639707437967No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7251.0955964578233142No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6931.0472390969262853No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6741.0185269138936743No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6731.0170157463656422No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6240.9429685374920664No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5420.8190528001934294No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5370.8114969625532686No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5350.8084746274972043No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5320.8039411249131079No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5230.7903406171608184No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4860.7344274186236286No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4760.7193157433433071No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4700.7102487381751141No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4610.6966482304228248No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4370.660380209750053No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4370.660380209750053No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4250.642246199413667No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4230.6392238643576026No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4230.6392238643576026No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4160.6286456916613775No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3980.6014446761567988No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3820.5772659957082842No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3780.5712213255961556No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3510.5304198023392873No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3420.5168192945869979No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3370.5092634569468372No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3340.5047299543627407No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3290.49717411672257983No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3050.4609060960498081No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3040.4593949285217759No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3030.4578837609937438No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3030.4578837609937438No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2910.43974975065735783No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2900.43823858312932573No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2810.4246380753770363No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2790.421615740320972No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2780.4201045727929399No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2770.4185934052649077No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2690.40650406504065045No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2670.40348172998458615No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2660.40197056245655394No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2650.4004593949285218No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2640.3989482274004896No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2610.39441472481639317No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2460.37174721189591076No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2330.35210203403149276No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2280.34454619639133194No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA2130.3218786834708496No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2130.3218786834708496No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2100.3173451808867531No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2080.31432284583068876No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1990.3007223380783994No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1960.2961888354943029No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.2856106627980778No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1860.2810771602139813No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1840.278054825157917No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1840.278054825157917No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1830.27654365762988486No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1770.26747665246169194No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1760.26596548493365973No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1730.2614319823495633No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1730.2614319823495633No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1670.25236497718137035No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1650.24934264212530602No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1620.24480913954120953No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1620.24480913954120953No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1580.23876446942908092No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.23876446942908092No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1570.23725330190104876No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1520.22969746426088794No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1500.22667512920482363No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1500.22667512920482363No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1500.22667512920482363No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1450.21911929156466287No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1430.21609695650859853No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1420.21458578898056638No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1410.21307462145253422No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1410.21307462145253422No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.21005228639646994No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1380.20854111886843776No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1370.2070299513404056No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.20249644875630912No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1340.20249644875630912No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1340.20249644875630912No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1320.1994741137002448No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.19040710853205187No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.1888959410040197No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1170.17680660077976246No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1140.17227309819566597No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1080.16320609302747305No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG1020.15413908785928007No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1010.15262792033124792No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.1496055852751836No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA980.14809441774715146No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT970.1465832502191193No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT970.1465832502191193No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC960.14507208269108712No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT960.14507208269108712No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA950.14356091516305497No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT950.14356091516305497No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.14053858010699066No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.13751624505092636No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC900.13600507752289417No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT900.13600507752289417No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC900.13600507752289417No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA890.13449390999486202No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA880.13298274246682987No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA870.13147157493879771No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.13147157493879771No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.13147157493879771No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA860.12996040741076556No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT850.12844923988273338No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.1254269048266691No Hit
ACATTGATGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG820.12391573729863693No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA810.12240456977060477No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC810.12240456977060477No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT810.12240456977060477No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC800.1208934022425726No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT800.1208934022425726No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.11635989965847614No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.11635989965847614No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC770.11635989965847614No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT760.11484873213044397No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC760.11484873213044397No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC740.11182639707437968No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA740.11182639707437968No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA730.11031522954634751No Hit
ACATTGATGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA720.10880406201831536No Hit
ACATTGATGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA720.10880406201831536No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT710.10729289449028319No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT710.10729289449028319No Hit
ACATTGATGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA700.10578172696225102No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.10427055943421888No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT680.10275939190618673No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC680.10275939190618673No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT670.10124822437815456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)