FastQCFastQC Report
Sun 21 May 2023
EGAF00007848432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46911
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA29286.241606446249281No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16483.513035322205879No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16073.4256357783888642No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8981.9142631792116986No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8491.8098100658694123No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8061.718147129671079No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7931.6904350791925136No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7781.65845963633263No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7391.5753234848969324No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7281.5518748267996845No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5741.2235936134382128No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5691.2129351324849182No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5521.1766962972437167No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5281.125535588667903No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5171.102086930570655No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5051.0765065762827482No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5021.0701114877107714No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4991.0637163991387948No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4901.0445311334228646No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4721.0061606019910043No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4260.9081025772206945No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4240.9038391848393766No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.8931807038860821No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4160.8867856153141054No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4050.8633369572168574No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3850.8207030334036793No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3790.8079128562597259No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3720.7929909829251135No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA3710.7908592867344546No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3600.7674106286372067No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3520.7503570591119353No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3500.7460936667306175No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3420.7290400972053462No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.7290400972053462No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3250.6928012619641448No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3200.6821427810108504No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3160.6736159962482147No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3090.6586941229136023No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3050.6501673381509667No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2990.6373771610070132No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2910.620323591481742No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2840.6054017181471296No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2810.5990066295751529No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2620.5585044019526337No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2530.5393191362367035No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.5371874400460447No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2490.5307923514740679No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2360.5030803009955022No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.4988169086141843No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2270.48389503527957195No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2250.47963164289825416No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2220.47323655432627737No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2160.46044637718232395No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2060.4391294152757349No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.4391294152757349No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2050.436997719085076No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.436997719085076No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.436997719085076No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1940.413549060987828No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1890.40289058003453343No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1890.40289058003453343No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1760.37517852955596764No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1670.3559932638400375No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1660.3538615676493786No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1600.3410713905054252No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1580.33680799812410733No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1580.33680799812410733No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1570.33467630193344844No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.32828121336147176No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1500.3197544285988361No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.3154910362175183No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1440.30696425145488265No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1420.3027008590735648No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.2941740743109292No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.2920423781202703No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1360.2899106819296114No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1350.2877789857389525No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1290.274988808594999No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1230.2621986314510456No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1190.25367184668841No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1180.2515401504977511No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1170.24940845430709216No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1160.24727675811643327No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.24727675811643327No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1150.24514506192577437No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.2387499733537976No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1120.2387499733537976No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1100.2344865809724798No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1070.22809149240050308No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1070.22809149240050308No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.22382810001918527No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.22169640382852634No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1010.21530131525654964No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1000.21316961906589071No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.2025111381125962No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT930.19824774573127837No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT900.19185265715930166No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA870.18545756858732493No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC870.18545756858732493No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.18332587239666603No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA850.1811941762060071No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT850.1811941762060071No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA850.1811941762060071No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA850.1811941762060071No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT830.1769307838246893No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT830.1769307838246893No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC820.1747990876340304No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT810.17266739144337148No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT800.1705356952527126No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT770.16414060668073588No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC760.16200891049007696No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA720.15348212572744133No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC710.1513504295367824No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA710.1513504295367824No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA710.1513504295367824No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.1513504295367824No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT700.1492187333461235No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC690.1470870371554646No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT680.1449553409648057No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA680.1449553409648057No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA640.13642855620217006No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT640.13642855620217006No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT640.13642855620217006No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT640.13642855620217006No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT620.13216516382085225No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.13216516382085225No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA610.13003346763019336No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC610.13003346763019336No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC600.12790177143953443No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT600.12790177143953443No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT590.12577007524887554No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA560.1193749866768988No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC550.1172432904862399No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT540.11511159429558099No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT540.11511159429558099No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA540.11511159429558099No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA540.11511159429558099No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA530.11297989810492208No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA530.11297989810492208No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT530.11297989810492208No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT520.11084820191426317No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.11084820191426317No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT520.11084820191426317No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC510.10871650572360426No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.10658480953294536No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA500.10658480953294536No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA480.10232141715162756No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA470.10018972096096865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)