FastQCFastQC Report
Sun 21 May 2023
EGAF00007848433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46911
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA28186.007119865276801No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14803.1549103621751824No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14753.1442518812218885No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9111.9419752296902646No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8231.7543859649122806No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8041.7138837372897615No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7961.6968301677644904No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7931.6904350791925136No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7891.6819082944298778No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7811.6648547249046064No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5831.242778879154143No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5311.1319306772398796No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5281.125535588667903No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5251.1191405000959265No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4901.0445311334228646No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4841.0317409562789113No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4731.008292298181663No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4630.9869753362750742No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4600.9805802477030975No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4490.9571315896058493No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4480.9549998934151904No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4240.9038391848393766No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4170.8889173115047644No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4130.8803905267421287No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4060.8654686534075164No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4050.8633369572168574No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4030.8590735648355395No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.8185713372130204No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3700.7887275905437956No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3500.7460936667306175No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA3370.7183816162520518No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3270.6970646543454627No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3030.6459039457696489No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3030.6459039457696489No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2920.6224552876724009No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2920.6224552876724009No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2800.596874933384494No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2780.5926115410031763No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2780.5926115410031763No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.5798213638592228No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2660.5670311867152693No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2630.5606360981432926No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2620.5585044019526337No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2610.5563727057619748No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2600.554241009571316No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2500.5329240476647268No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2480.528660655283409No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2460.5243972629020912No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2420.5158704781394556No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2410.5137387819487966No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2290.4881584276608898No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2200.4689731619449596No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2170.4625780733729829No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2050.436997719085076No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2030.4327343267037582No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1990.4242075419411226No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.4007588838438746No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1860.39649549146255675No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1830.39010040289058007No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1830.39010040289058007No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.3815736181279444No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.37091513717464986No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1640.3495981752680608No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1620.34533478288674296No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1590.3389396943147663No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.33680799812410733No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1560.33254460574278955No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1520.3240178209801539No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1520.3240178209801539No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1510.321886124789495No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1490.3176227324081772No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1470.3133593400268594No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1440.30696425145488265No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1440.30696425145488265No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1420.3027008590735648No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1400.298437466692247No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1400.298437466692247No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.2899106819296114No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1340.28564728954829355No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1320.28138389716697576No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1310.27925220097631687No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.2621986314510456No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1210.2579352390697278No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1190.25367184668841No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1150.24514506192577437No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1140.24301336573511542No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1120.2387499733537976No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.2344865809724798No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1100.2344865809724798No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1070.22809149240050308No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.22169640382852634No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1000.21316961906589071No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA980.20890622668457293No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA970.206774530493914No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA970.206774530493914No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT960.2046428343032551No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT960.2046428343032551No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT940.2003794419219373No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA930.19824774573127837No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA920.19611604954061948No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC900.19185265715930166No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT890.18972096096864274No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT880.18758926477798382No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT880.18758926477798382No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.18545756858732493No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT870.18545756858732493No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.18332587239666603No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG850.1811941762060071No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT840.17906248001534822No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT830.1769307838246893No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA830.1769307838246893No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC820.1747990876340304No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA800.1705356952527126No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT790.16840399906205367No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT790.16840399906205367No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT770.16414060668073588No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC760.16200891049007696No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT730.15561382191810022No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.15561382191810022No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT720.15348212572744133No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC720.15348212572744133No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA710.1513504295367824No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA710.1513504295367824No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT710.1513504295367824No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT710.1513504295367824No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC700.1492187333461235No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.1470870371554646No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT680.1449553409648057No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT680.1449553409648057No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.1449553409648057No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA680.1449553409648057No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC660.14069194858348788No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA630.13429686001151117No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC620.13216516382085225No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA620.13216516382085225No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.13216516382085225No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.12790177143953443No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT590.12577007524887554No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG580.12363837905821663No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC570.12150668286755771No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA570.12150668286755771No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.12150668286755771No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA560.1193749866768988No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCT540.11511159429558099No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA540.11511159429558099No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC530.11297989810492208No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT530.11297989810492208No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.11084820191426317No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.11084820191426317No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.10871650572360426No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA500.10658480953294536No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT500.10658480953294536No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.10445311334228646No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCT490.10445311334228646No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA490.10445311334228646No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.10232141715162756No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.10018972096096865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)