FastQCFastQC Report
Sun 21 May 2023
EGAF00007848434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65341
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG24583.761803461838662No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG23983.669977502639996No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG17832.728761420853675No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG15902.433387918764635No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13962.1364839840222833No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT13011.9910928819577294No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG12921.9773189880779296No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12851.966605959504752No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12111.8533539431597312No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11991.834988751319998No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9791.498293567591558No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9341.429424098192559No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9241.414119771659448No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8761.3406590043005158No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8541.3069894859276718No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT7521.1508853552899405No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7371.1279288654902742No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7321.1202767022237188No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7311.1187462695704076No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA7131.0911984818108078No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7121.0896680491574968No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7101.0866071838508746No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6721.0284507430250531No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5210.7973554123750785No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5150.788172816455212No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4960.7590945960423012No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4900.7499120001224346No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4790.7330772409360126No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4570.6994077225631685No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4550.6963468572565464No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA4490.68716426133668No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4460.6825729633767467No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4330.6626773388837024No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4220.6458425796972804No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4150.6351295511241027No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4120.6305382531641696No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA4070.6228860898976141No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3970.6075817633645031No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3940.6029904654045699No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3880.5938078694847033No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3700.5662600817251037No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3600.5509557551919928No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA3510.5371818613121929No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3380.5172862368191488No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3250.49739061232610454No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3120.47749498783306044No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3090.47290368987312714No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3090.47290368987312714No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2950.4514776327267719No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2880.44076460415359425No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2860.437703738846972No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2820.43158200823372767No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2730.4178081143539279No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2720.41627768170061674No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2690.4116863837406835No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2620.4009733551675059No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2560.39179075924763934No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2560.39179075924763934No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2550.39026032659432824No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2550.39026032659432824No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2520.38566902863439495No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2470.37801686536783946No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2470.37801686536783946No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2430.3718951347545951No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2300.35199951026155096No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2290.35046907760823987No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2180.33363431842181784No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2120.3244517225019513No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2100.3213908571953291No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2080.3183299918887069No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2070.3167995592353958No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2040.3122082612754626No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2020.3091473959688404No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.3091473959688404No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2020.3091473959688404No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2010.3076169633155293No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2000.3060865306622182No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.29537350208904056No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.2632344163695077No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1710.2617039837161966No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1680.2571126857562633No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1680.2571126857562633No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1650.25252138779633004No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1650.25252138779633004No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1640.2509909551430189No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1610.24639965718308565No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1590.24333879187646346No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1560.23874749391653022No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1540.23568662860990802No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1540.23568662860990802No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1490.22803446534335256No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1480.22650403269004146No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1420.21732143677017493No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1350.2066084081969973No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1350.2066084081969973No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1320.202017110237064No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1300.1989562449304418No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1290.19742581227713074No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1260.19283451431719748No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1240.1897736490105753No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1240.1897736490105753No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1210.185182351050642No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1190.18212148574401982No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1180.18059105309070875No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1180.18059105309070875No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1160.17753018778408658No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1140.17446932247746438No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1130.17293888982415329No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1130.17293888982415329No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1120.1714084571708422No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1110.1698780245175311No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1110.1698780245175311No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1100.16834759186422002No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1060.16222586125097566No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.16069542859766456No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA1040.15916499594435346No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1010.1545736979844202No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT990.151512832677798No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA950.14539110206455366No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT930.14233023675793147No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT920.14079980410462037No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA880.134678073491376No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC870.13314764083806493No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT870.13314764083806493No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC860.13161720818475384No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC840.12855634287813164No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTG820.12549547757150945No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA820.12549547757150945No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT790.12090417961157619No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC790.12090417961157619No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA790.12090417961157619No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA770.11784331430495401No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC760.11631288165164291No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC750.11478244899833182No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC750.11478244899833182No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT750.11478244899833182No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTG740.11325201634502073No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA740.11325201634502073No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.11172158369170966No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA730.11172158369170966No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT730.11172158369170966No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT700.10713028573177638No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT700.10713028573177638No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA700.10713028573177638No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT700.10713028573177638No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC690.10559985307846528No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.10559985307846528No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA690.10559985307846528No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA680.10406942042515419No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG680.10406942042515419No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT680.10406942042515419No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)