FastQCFastQC Report
Sun 21 May 2023
EGAF00007848435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65341
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG22633.463369094442999No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG22353.4205169801502886No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG16012.4502226779510567No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG15812.419614024884835No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT15412.3583967187523913No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT14882.2772837881269035No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT13482.0630232166633506No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12981.9865015839977962No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11631.779893175800799No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG11401.7446932247746436No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10071.5411456818842686No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9801.4998240002448693No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8161.2488330451018503No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7951.2166939593823174No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7701.1784331430495403No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA7571.158537518556496No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7451.140172326716763No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7441.1386418940634517No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7381.1294592981435851No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT7351.124868000183652No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7341.123337567530341No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA6741.0315116083316753No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6691.02385944506512No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5410.8279640654413003No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT5180.7927641144151452No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT5030.7698076246154788No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4980.7621554613489233No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4970.7606250286956122No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4950.7575641633889901No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4930.7545032980823678No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4450.6810425307234356No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4330.6626773388837024No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA4210.6443121470439693No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4210.6443121470439693No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4180.639720849084036No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4100.6274773878575474No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4060.621355657244303No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3880.5938078694847033No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3750.5739122449916592No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3730.570851379685037No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA3680.5631992164184815No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3650.5586079184585482No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3620.554016620498615No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3610.5524861878453038No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3520.538712293965504No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3510.5371818613121929No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3350.5126949388592156No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3150.4820862857929937No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3050.46678195925988275No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2930.44841676742014963No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2840.43464287354034986No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2770.42392984496717223No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.41474724904730564No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2580.3948516245542615No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2560.39179075924763934No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2550.39026032659432824No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2510.38413859598108385No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2500.38260816332777275No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.38260816332777275No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2480.3795472980211506No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2440.3734255674079062No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2420.370364702101284No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2420.370364702101284No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2400.36730383679466183No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2380.36424297148803964No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2380.36424297148803964No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2350.3596516735281064No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2330.3565908082214842No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.353529942914862No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2260.34587777964830657No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2100.3213908571953291No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2080.3183299918887069No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2030.3106778286221515No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2020.3091473959688404No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2000.3060865306622182No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1990.3045560980089071No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1880.28772133882248513No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1880.28772133882248513No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1830.2800691755559297No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1780.2724170122893742No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1750.2678257143294409No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1750.2678257143294409No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1730.2647648490228187No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1710.2617039837161966No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1680.2571126857562633No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1620.24793008983639678No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1580.24180835922315239No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1540.23568662860990802No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1510.23109533064997476No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1510.23109533064997476No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1460.22344316738341932No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1450.2219127347301082No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1450.2219127347301082No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1440.22038230207679713No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1410.21579100411686383No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1400.21426057146355276No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1390.21273013881024164No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1390.21273013881024164No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1390.21273013881024164No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1350.2066084081969973No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1240.1897736490105753No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1210.185182351050642No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1190.18212148574401982No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1160.17753018778408658No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1120.1714084571708422No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1100.16834759186422002No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1090.16681715921090892No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1090.16681715921090892No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1060.16222586125097566No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT1030.1576345632910424No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1020.1561041306377313No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1020.1561041306377313No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1020.1561041306377313No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.1545736979844202No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA990.151512832677798No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA990.151512832677798No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA980.14998240002448693No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.14998240002448693No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA960.14692153471786473No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC950.14539110206455366No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC950.14539110206455366No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA940.14386066941124256No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC930.14233023675793147No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC900.1377389387979982No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT900.1377389387979982No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG890.1362085061446871No Hit
TCGGCCTCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA880.134678073491376No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC870.13314764083806493No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA860.13161720818475384No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC860.13161720818475384No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTG850.13008677553144274No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA850.13008677553144274No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT850.13008677553144274No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.12855634287813164No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG840.12855634287813164No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA820.12549547757150945No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA810.12396504491819839No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC800.12243461226488729No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT800.12243461226488729No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.12090417961157619No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT790.12090417961157619No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT780.11937374695826511No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTG760.11631288165164291No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT760.11631288165164291No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT740.11325201634502073No Hit
TCGGCCTCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA740.11325201634502073No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCT690.10559985307846528No Hit
TCGGCCTCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA670.1025389877718431No Hit
TCGGCCTCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA670.1025389877718431No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT660.101008555118532No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC660.101008555118532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)