FastQCFastQC Report
Sun 21 May 2023
EGAF00007848446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42353
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG13423.1686067102684583No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12382.9230514957618117No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG11992.830968290321819No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10772.5429131348428684No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9262.1863858522418718No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8862.091941538970085No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8471.9998583335300923No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7721.8227752461454916No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7121.6811087762378107No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6801.605553325620381No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6671.57485892380705No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6371.50402568885321No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5241.2372205038604114No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5051.1923594550563124No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4641.0955540339527308No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4331.0223596911670956No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4020.9491653483814606No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4000.9444431327178713No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3980.9397209170542817No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3980.9397209170542817No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3870.9137487309045403No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3730.8806932212594148No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3690.8712487899322363No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3610.8523599272778787No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.835832172455316No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3370.7956933393148066No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3160.7461100748471182No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3110.7343045356881449No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3060.7224989965291715No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3010.7106934573701982No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.6941657025476353No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2860.6752768398932779No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2790.6587490850707152No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2750.6493046537435365No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2670.630415791089179No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2540.5997213892758482No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2520.594999173612259No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2490.5879158501168749No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2480.5855547422850801No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2440.5761103109579014No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2400.5666658796307228No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2350.5548603404717493No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.5454159091445706No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2280.5383325856491866No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2270.535971477817392No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2210.5218048308266239No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2190.5170826151630346No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2190.5170826151630346No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2170.5123603994994451No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2170.5123603994994451No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2150.5076381838358558No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2150.5076381838358558No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2110.49819375250867703No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2010.4745826741907303No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1970.4651382428635516No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1920.4533327037045782No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1850.43680494888201543No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1690.39902722357330056No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1650.38958279224612186No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1580.3730550374235591No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1570.37069392959176445No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1540.36361060609638046No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.36124949826458574No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1530.36124949826458574No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1420.3352773121148443No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1340.31638844946048683No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1330.31402734162869217No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.30458291030151347No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1270.2998606946379241No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.27624961631997735No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1170.27624961631997735No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1150.271527400656388No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1150.271527400656388No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1130.26680518499279865No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.26444407716100393No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1070.25263853800203057No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1070.25263853800203057No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1050.24791632233844121No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1050.24791632233844121No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1040.2455552145066465No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1030.24319410667485186No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA1030.24319410667485186No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.24083299884305717No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.23847189101126245No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.23138856751587847No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC960.2266663518522891No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT960.2266663518522891No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT930.21958302835690507No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA920.21722192052511038No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT920.21722192052511038No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC910.2148608126933157No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT900.21249970486152103No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA900.21249970486152103No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG890.21013859702972634No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.205416381366137No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC870.205416381366137No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT860.2030552735343423No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT850.20069416570254764No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC840.19833305787075295No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.1912497343753689No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA800.18888862654357425No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG790.18652751871177956No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA790.18652751871177956No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC780.1841664108799849No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC780.1841664108799849No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC760.1794441952163955No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA760.1794441952163955No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.1794441952163955No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA750.17708308738460085No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT750.17708308738460085No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA740.1747219795528062No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC740.1747219795528062No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.1723608717210115No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG730.1723608717210115No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA730.1723608717210115No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.1723608717210115No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA730.1723608717210115No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.16999976388921684No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT720.16999976388921684No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.16763865605742215No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA710.16763865605742215No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA710.16763865605742215No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA700.16527754822562746No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.1629164403938328No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC690.1629164403938328No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.1629164403938328No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.1629164403938328No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.1629164403938328No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.1605553325620381No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT670.15819422473024342No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.15819422473024342No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA660.15583311689844875No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA660.15583311689844875No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC650.15347200906665406No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT640.1511109012348594No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA640.1511109012348594No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA640.1511109012348594No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT630.14874979340306474No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC620.14638868557127002No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.14402757773947536No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC610.14402757773947536No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT560.13222203858050197No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA550.1298609307487073No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.1298609307487073No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT550.1298609307487073No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC530.12513871508511792No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC530.12513871508511792No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA530.12513871508511792No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA520.12277760725332325No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT520.12277760725332325No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC520.12277760725332325No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.12041649942152859No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT510.12041649942152859No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.11569428375793923No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.11569428375793923No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11097206809434987No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC460.10861096026255519No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT460.10861096026255519No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG450.10624985243076052No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.10388874459896584No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA440.10388874459896584No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.10388874459896584No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT440.10388874459896584No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT430.10152763676717115No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT430.10152763676717115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)