FastQCFastQC Report
Sun 21 May 2023
EGAF00007848455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54861
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA23024.196059131259No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA16693.0422340095878675No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT12232.2292703377627094No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11742.139953701172053No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11382.0743333151054486No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10461.9066367729352365No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG9911.8063834053334793No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT8771.5985855161225644No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8121.4801042635023058No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA8071.4709903209930553No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7381.3452179143653962No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA7131.2996482018191429No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6851.2486101237673393No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6611.2048631997229362No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6411.1684074296859335No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCAAGCGTTG6241.1374200251544813No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6191.1283060826452307No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA6181.1264832941433804No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5701.0389894460545743No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5390.9824830024972203No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5300.9660779059805691No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA4990.909571462423215No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4560.8311915568436594No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4540.8275459798399591No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4520.823900402836259No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4460.812963671825158No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4430.8074953063196078No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4410.8038497293159076No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCAAGCGTTG4260.7765079017881555No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT4190.7637483822752046No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3890.7090647272197007No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3630.6616722261715973No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3620.6598494376697471No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3590.6543810721641968No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3580.6525582836623467No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3360.6124569366216438No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3310.6033429941123931No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3240.5905834745994422No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2910.5304314540383879No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2870.5231403000309874No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2790.5085579920161865No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2770.5049124150124861No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2760.5030896265106359No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA2700.49215289549953517No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT2670.4866845299939848No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2660.4848617414921347No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2620.47757058748473413No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2610.475747798982884No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2560.4666338564736334No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2470.45022875995698214No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2330.4247097209310804No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2240.4083046244144292No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2230.406481835912579No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2210.4028362589088788No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2210.4028362589088788No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCAAGCGTTG2150.391899527897778No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.39007673939592785No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA2110.3846083738903775No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2070.377317219882977No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2060.3754944313811268No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1980.3609121233663258No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1960.35726654636262556No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1940.3536209693589253No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1850.3372158728422741No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1800.3281019303330235No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1790.32627914183117335No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1720.31351962231822245No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1710.3116968338163723No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1690.30805125681267204No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1640.2989373143034214No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1610.293468948797871No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.29164616029602086No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1590.2898233717941707No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1580.2880005832923206No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1570.28617779479047045No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1560.2843550062886203No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1550.2825322177867702No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1540.28070942928492004No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1530.27888664078306996No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAAGGGGCGAGCGTTG1500.27341827527751955No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1420.2588359672627185No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1410.2570131787608684No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1390.2533676017571681No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1360.24789923625161775No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1330.24243087074606734No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCATGCT1290.23513971673866682No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAAGGGGCGAGCGTTG1290.23513971673866682No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT1270.23149413973496655No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1250.2278485627312663No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCAAGCGTTG1240.22602577422941617No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1220.2223801972257159No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATGCGGGGGGTGCGAGCGTTA1180.21508904321831537No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1170.21326625471646526No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCT1160.21144346621461513No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1150.20962067771276496No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1140.20779788921091485No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1140.20779788921091485No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1110.20232952370536444No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1100.20050673520351434No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1080.1968611581998141No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1070.19503836969796393No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1060.19321558119611382No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.19139279269426368No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1040.18957000419241354No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.18227885018501302No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA990.1804560616831629No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCT980.17863327318131278No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT980.17863327318131278No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT950.17316490767576237No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT930.16951933067206212No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA930.16951933067206212No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCT910.16587375366836185No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTA880.16040538816281147No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG880.16040538816281147No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC850.15493702265726106No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG850.15493702265726106No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCT830.1512914456535608No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCT830.1512914456535608No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT830.1512914456535608No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCT810.14764586864986054No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA810.14764586864986054No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATGCGGGGGGTGCGAGCGTTA800.14582308014801043No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT800.14582308014801043No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT780.14217750314431016No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA760.1385319261406099No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA740.13488634913690964No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC740.13488634913690964No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT700.12759519512950912No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.12577240662765898No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC690.12577240662765898No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA690.12577240662765898No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC670.12212682962395872No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT660.12030404112210862No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT660.12030404112210862No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA660.12030404112210862No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC650.11848125262025846No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.11665846411840836No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCT640.11665846411840836No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC640.11665846411840836No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCATGCT630.1148356756165582No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAAGGGGCGAGCGTTG630.1148356756165582No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTA610.11119009861285795No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA610.11119009861285795No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT600.10936731011100782No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT600.10936731011100782No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC590.10754452160915769No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCATGCT590.10754452160915769No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCC590.10754452160915769No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAAGGTGCGAGCGTTG580.10572173310730756No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.10572173310730756No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC580.10572173310730756No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA580.10572173310730756No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC570.10389894460545743No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.10389894460545743No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT570.10389894460545743No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC560.1020761561036073No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCATGCT560.1020761561036073No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA560.1020761561036073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)