FastQCFastQC Report
Sun 21 May 2023
EGAF00007848456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48682
Sequences flagged as poor quality0
Sequence length250
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA27735.696150527915862No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA19073.9172589458115934No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA16313.350314284540487No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA11092.278049381701656No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA11042.2677786450844257No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA7801.6022349122879094No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA7671.5755309970831108No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA6351.304383550388234No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5471.1236185859249825No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA5081.043506840310587No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4991.0250195143995726No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4971.0209112197526806No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4951.0168029251057886No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4921.0106404831354505No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA4490.9223121482272709No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGGGCAAGCGTTA4360.8956082330224723No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4340.8914999383755803No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4180.8586335812004436No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4170.8565794338769978No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4120.8463086972597674No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3860.7929008668501705No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT3790.7785218355860483No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT3720.7641428043219259No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3610.7415471837640196No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT3600.7394930364405735No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3510.7210057105295592No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3490.7168974158826671No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3470.712789121235775No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC3210.659381290826178No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.6450022595620558No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3110.6388398175917177No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3100.6367856702682717No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3050.6265149336510414No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3040.6244607863275954No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC3030.6224066390041494No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2980.6121359023869192No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2930.601865165769689No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT2910.597756871122797No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2840.5833778398586746No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2810.5772153978883365No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2780.5710529559179984No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACT2750.5648905139476603No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC2730.5607822193007683No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGGGCAAGCGTTA2710.5566739246538762No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.5443490407132No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2620.5381865987428619No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2580.5299700094490777No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2570.5279158621256317No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2450.5032660942442791No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTGTGTCACGAGCGTTA2390.49094121030360294No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2390.49094121030360294No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2290.4703997370691426No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG2270.4662914424222505No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2210.4539665584815743No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2210.4539665584815743No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2200.45191241115812825No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2180.44780411651123614No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT2160.44369582186434414No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2090.4293167906002218No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2090.4293167906002218No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.4231543486298837No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2050.4211002013064377No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACT1950.40055872807197734No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1940.39850458074853123No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1940.39850458074853123No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1930.39645043342508524No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACT1920.39439628610163924No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACT1840.3779631075140709No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1840.3779631075140709No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1830.37590896019062486No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1820.37385481286717887No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACT1810.3718006655437328No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACT1790.36769237089684076No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.36769237089684076No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1790.36769237089684076No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG1790.36769237089684076No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1780.3656382235733947No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1780.3656382235733947No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1780.3656382235733947No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1730.3553674869561645No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1700.3492050449858264No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1690.34715089766238033No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTGTGTCACGAGCGTTA1660.3409884556920422No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1650.33893430836859617No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1650.33893430836859617No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1620.33277186639825806No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1610.33071771907481207No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1610.33071771907481207No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1580.32455527710447396No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.31017624584035164No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1500.3081220985169056No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTGTGTCACGAGCGTTA1430.2937430672527834No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1420.2916889199293373No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACT1410.2896347726058913No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1400.28758062528244527No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1400.28758062528244527No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACT1400.28758062528244527No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1400.28758062528244527No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1380.2834723306355532No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1380.2834723306355532No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1370.28141818331210716No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1330.273201594018323No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1330.273201594018323No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACT1320.271147446694877No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1320.271147446694877No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1310.26909329937143095No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1280.2629308574010928No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1270.26087671007764673No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1260.25882256275420074No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACT1240.25471426810730863No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACT1240.25471426810730863No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACT1210.24855182613697052No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1200.24649767881352452No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1160.23828108951974036No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC1150.2362269421962943No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1140.2341727948728483No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1140.2341727948728483No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1110.22801035290251018No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC1110.22801035290251018No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.22595620557906412No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC1090.22390205825561807No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTA1070.21979376360872602No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1060.21773961628527996No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1050.21568546896183396No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1040.2136313216383879No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA950.19514399572737356No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT940.19308984840392754No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT920.18898155375703546No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC910.18692740643358943No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT900.18487325911014338No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACT880.1807649644632513No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC850.1746025224929132No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT840.17254837516946714No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACT840.17254837516946714No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA830.1704942278460211No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTGTGTCACGAGCGTTA810.16638593319912903No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT810.16638593319912903No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC800.164331785875683No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA790.16227763855223698No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG780.16022349122879093No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACT770.1581693439053449No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTCGATACCCGCACT750.1540610492584528No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATTCGAGCGTTA750.1540610492584528No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACT740.15200690193500677No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA710.14584445996466866No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG710.14584445996466866No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.14379031264122263No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.14379031264122263No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTC690.1417361653177766No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT680.13968201799433055No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACT670.13762787067088453No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACC660.1355737233474385No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT650.13351957602399245No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA640.1314654287005464No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT620.12735713405365431No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTCGATACCCGCACC590.12119469208331621No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT590.12119469208331621No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA570.11708639743642416No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC520.10681566081919396No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATCCCCACACT520.10681566081919396No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTA510.10476151349574792No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTC500.10270736617230188No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATCCCCACACT500.10270736617230188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)