FastQCFastQC Report
Sun 21 May 2023
EGAF00007848481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39578
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11342.865228157056951No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9902.501389660922735No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9532.407903380666027No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7651.9328920107130225No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7071.7863459497700744No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6551.6549598261660519No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5991.5134670776694124No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5921.4957804841073323No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5211.3163878922633785No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5091.2860680175855272No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4741.1976350497751276No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4691.1850017686593561No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4531.1445752690888877No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4521.1420486128657334No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4511.1395219566425792No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4421.1167820506341908No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4361.101622113295265No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4181.056142301278488No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4091.0334023952700995No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3930.9929758956996311No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3850.9727626459143969No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3740.9449694274596998No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3720.9399161150133913No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3660.9247561776744656No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3610.9121228965586943No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3290.8312698974177574No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3200.8085299914093689No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.7933700540704431No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3070.7756834605083632No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3010.7605235231694376No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2850.7200970235989691No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2780.7024104300368892No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.7024104300368892No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.6594572742432665No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2610.6594572742432665No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2570.6493506493506493No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2550.6442973369043408No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2520.6367173682348779No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2510.6341907120117237No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2470.6240840871191066No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2380.6013441811107181No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2370.5988175248875638No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2290.5786042751023296No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.5710243064328667No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2240.5659709939865581No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2240.5659709939865581No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2230.5634443377634039No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2170.5482844004244782No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1970.49775127596139274No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1930.4876446510687755No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1930.4876446510687755No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1890.4775380261761585No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1880.4750113699530042No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1860.46995805750669567No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1850.4674314012835414No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1810.4573247763909242No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1750.44216483905199855No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1740.43963818282884437No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.4371115266056901No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1720.43458487038253574No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1670.4219515892667644No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1650.4168982768204558No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1640.4143716205973015No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1620.409318308150993No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1570.39668502703522157No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.38657840214260447No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1490.37647177724998737No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.36636515235737027No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1440.363838496134216No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1430.3613118399110617No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1330.3360452776795189No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1320.33351862145636463No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1320.33351862145636463No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.33351862145636463No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.32088534034059324No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1260.31835868411743895No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1250.3158320278942847No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1240.31330537167113043No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.31330537167113043No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1220.3082520592248219No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1180.29814543433220475No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1150.29056546566274194No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1120.2829854969932791No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1110.2804588407701248No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.27540552832381626No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1070.27035221587750774No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.2627722472080449No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.25519227853858206No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA940.2375056849765021No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT920.23245237253019355No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA900.227399060083885No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA890.2248724038607307No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA890.2248724038607307No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT870.21981909141442219No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT870.21981909141442219No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC870.21981909141442219No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC850.21476577896811358No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC840.21223912274495935No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT830.20971246652180506No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT820.20718581029865074No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT810.2046591540754965No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT800.20213249785234222No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT780.19707918540603367No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC770.19455252918287938No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT770.19455252918287938No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA760.19202587295972512No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC750.18949921673657083No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT750.18949921673657083No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.18949921673657083No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA740.18697256051341654No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC730.18444590429026228No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.181919248067108No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC700.17686593562079944No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT700.17686593562079944No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.17433927939764515No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC690.17433927939764515No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.17433927939764515No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA690.17433927939764515No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.1718126231744909No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC660.16675931072818231No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC660.16675931072818231No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC630.15917934205871948No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT610.15412602961241095No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA590.14907271716610238No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.14654606094294811No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT580.14654606094294811No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT570.14401940471979383No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA570.14401940471979383No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT540.136439436050331No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA540.136439436050331No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT530.1339127798271767No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA530.1339127798271767No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC530.1339127798271767No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC520.13138612360402244No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA520.13138612360402244No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT510.12885946738086815No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.12885946738086815No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT500.1263328111577139No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC500.1263328111577139No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.1263328111577139No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.1263328111577139No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT490.12380615493455961No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT490.12380615493455961No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT490.12380615493455961No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT490.12380615493455961No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA480.12127949871140534No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.11875284248825105No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT470.11875284248825105No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT460.11622618626509677No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT460.11622618626509677No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC460.11622618626509677No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT460.11622618626509677No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT450.1136995300419425No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.1136995300419425No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA440.11117287381878821No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA440.11117287381878821No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT440.11117287381878821No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.11117287381878821No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.11117287381878821No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA430.10864621759563393No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA430.10864621759563393No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA430.10864621759563393No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC420.10611956137247967No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT420.10611956137247967No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT420.10611956137247967No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCCGGCGTTA420.10611956137247967No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG420.10611956137247967No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC410.10359290514932537No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT400.10106624892617111No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA400.10106624892617111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)