FastQCFastQC Report
Sun 21 May 2023
EGAF00007848492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31494
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14134.486568870261002No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10773.4196989902838633No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9072.8799136343430494No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8412.6703499079189688No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6652.11151330412142No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6602.095637264240808No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5521.7527148028195847No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5201.6511081475836666No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5111.6225312757985648No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4381.390741093541627No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4371.3875658855655046No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4261.3526385978281577No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3871.2288054867593827No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3751.1907029910459135No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3731.1843525750936688No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3511.114497999618975No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3401.0795707118816282No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3221.0224169683114244No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3181.0097161364069347No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3020.9589128087889757No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2950.9366863529561186No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2840.9017590652187718No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2780.8827078173620372No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2650.8414301136724456No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2590.822378865815711No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2570.816028449863466No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.8001524099828539No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2340.74299866641265No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2280.7239474185559154No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2190.6953705467708134No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2130.6763192989140788No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.6763192989140788No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC2130.6763192989140788No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2120.6731440909379565No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2090.663618467009589No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2080.6604432590334667No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.6572680510573443No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1900.6032895154632628No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1890.6001143074871403No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.596939099511018No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1870.5937638915348956No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1810.5747126436781609No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1800.5715374357020385No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1690.5366101479646916No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1630.5175589001079571No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1610.5112084841557122No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1460.4635803645138757No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1450.46040515653775327No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1420.4508795326093859No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1420.4508795326093859No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1400.44452911665714107No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1400.44452911665714107No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1390.4413539086810186No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1370.4350034927287737No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1350.4286530767765289No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1320.41912745284816155No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1320.41912745284816155No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1280.4064266209436718No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1270.40325141296754935No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1200.3810249571346923No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1170.371499333206325No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1130.35879850130183527No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1080.34292246142122307No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1060.33657204546897823No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1050.3333968374928558No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1040.33022162951673334No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.3270464215406109No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1030.3270464215406109No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.3270464215406109No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1020.32387121356448845No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1010.3206960055883661No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT990.3143455896361212No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT970.3079951736838763No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA950.3016447577316314No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC940.298469549755509No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA940.298469549755509No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA930.29529434177938657No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.29529434177938657No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC920.2921191338032641No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC910.2889439258271417No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA910.2889439258271417No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC890.2825935098748968No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.2825935098748968No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT820.26036705404203975No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT810.2571918460659173No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC800.2540166380897949No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC790.25084143011367244No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT770.24449101416142757No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC760.24131580618530513No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.2381405982091827No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.2381405982091827No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA750.2381405982091827No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT740.2349653902330603No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC720.2286149742808154No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA700.22226455832857053No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT700.22226455832857053No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACC680.21591414237632564No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT670.2127389344002032No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA660.20956372642408078No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.20956372642408078No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC640.2032133104718359No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT630.20003810249571347No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC610.1936876865434686No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC610.1936876865434686No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT600.19051247856734616No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA590.1873372705912237No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT570.18098685463897884No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.1778116466628564No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.1778116466628564No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT540.17146123071061153No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA540.17146123071061153No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC540.17146123071061153No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT530.16828602273448912No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.16828602273448912No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.16828602273448912No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC510.16193560678224422No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT510.16193560678224422No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA510.16193560678224422No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA510.16193560678224422No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.1587603988061218No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.1587603988061218No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT490.15558519082999936No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA490.15558519082999936No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC480.15240998285387694No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA480.15240998285387694No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT480.15240998285387694No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.15240998285387694No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC470.1492347748777545No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.1492347748777545No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACC450.14288435892550963No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT450.14288435892550963No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.14288435892550963No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC430.13653394297326474No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT420.13335873499714232No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.13335873499714232No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACC420.13335873499714232No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.13018352702101987No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA410.13018352702101987No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT410.13018352702101987No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT410.13018352702101987No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA410.13018352702101987No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC400.12700831904489746No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.12700831904489746No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA400.12700831904489746No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA390.12383311106877501No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA390.12383311106877501No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC390.12383311106877501No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA390.12383311106877501No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT380.12065790309265256No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT370.11748269511653014No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG370.11748269511653014No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.11748269511653014No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA360.1143074871404077No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA360.1143074871404077No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT350.11113227916428527No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT350.11113227916428527No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT350.11113227916428527No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT340.10795707118816282No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC340.10795707118816282No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG340.10795707118816282No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG340.10795707118816282No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT340.10795707118816282No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC340.10795707118816282No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA340.10795707118816282No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT330.10478186321204039No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT330.10478186321204039No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA320.10160665523591796No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT320.10160665523591796No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG320.10160665523591796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)