FastQCFastQC Report
Sun 21 May 2023
EGAF00007848495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91243
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21932.403472047170742No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20672.2653792619707813No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG20012.193044945913659No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG19922.1831811755422335No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG15301.676840963142378No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG15151.6604013458566684No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14251.561763642142411No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14081.5431320758852733No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT13891.522308560656708No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13701.5014850454281425No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT13331.4609339894567255No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG12811.403943316199599No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG12581.3787359030281774No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA12331.3513365408853282No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11961.3107854849139111No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA11871.3009217145424854No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA11591.270234428942494No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA10701.1726926997139506No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT10221.12008592439968No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT10111.1080302050568263No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9701.0630952511425535No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9581.049943557313986No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9120.999528730971143No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8660.9491139046283003No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8330.9129467465997392No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8220.9008910272568855No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8190.8976031037997436No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7920.8680117926854664No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7810.8559560733426126No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7420.8132130683997676No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7400.8110211194283398No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6980.7649901910283529No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6790.7441666757997875No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6690.7332069309426477No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6690.7332069309426477No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT6620.7255351095426499No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6530.7156713391712242No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6510.7134793901997961No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5610.6148416864855386No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA5430.5951141457426871No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA5340.5852503753712613No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5280.5786745284569775No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT5040.5523711407998422No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4900.5370274979998466No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4750.520587880714137No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA4740.519491906228423No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4680.5129160593141392No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA4590.5030522889427135No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4470.4899005951141458No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4330.4745569523141501No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4320.4734609778284361No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4170.4570213605427266No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA4110.45044551362844276No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4090.4482535646570147No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4070.44606161568558683No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4010.439485768771303No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT3980.4361978453141611No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3880.4252381004570214No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3850.42195017699987947No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3710.4066065341998838No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3630.39783873831417205No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3630.39783873831417205No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3620.3967427638284581No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT3560.3901669169141742No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA3480.38139912102846246No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA3470.3803031465427485No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA3320.36386352925703885No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3310.36276755477132494No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3080.33756014159990355No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA3020.3309842946856197No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3010.32988832019990577No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT3000.32879234571419175No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2820.30906480497134026No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC2810.3079688304856263No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2770.3035849325427704No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2750.3013929835713425No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2640.28933726422848877No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2610.28604934077134686No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2420.26522582554278135No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2410.26412985105706743No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC2350.25755400414278357No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2300.25207413171421367No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCC2300.25207413171421367No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2300.25207413171421367No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2260.2476902337713578No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT2220.24330633582850195No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2200.24111438685707398No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2160.23673048891421805No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2110.2312506164856482No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2080.22796269302850627No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT2060.22577074405707837No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2020.22138684611422246No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2000.2191948971427945No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1940.2126190502285107No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1920.21042710125708272No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1890.20713917779994082No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC1880.20604320331422685No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1880.20604320331422685No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1860.20385125434279888No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCC1830.20056333088565698No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1810.19837138191422904No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1780.1950834584570871No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1690.18521968808566136No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1680.1841237135999474No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCC1660.18193176462851943No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1660.18193176462851943No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1660.18193176462851943No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1630.17864384117137752No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1610.17645189219994958No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1560.1709720197713797No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1540.16878007079995178No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1540.16878007079995178No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1480.16220422388566794No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCC1440.15782032594281206No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1410.15453240248567013No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1340.14686058108567235No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1310.1435726576285304No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1280.14028473417138848No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1280.14028473417138848No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT1260.13809278519996054No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1260.13809278519996054No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1250.13699681071424658No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCC1250.13699681071424658No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCC1240.1359008362285326No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1200.1315169382856767No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG1200.1315169382856767No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.1315169382856767No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1200.1315169382856767No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1180.12932498931424877No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCC1170.1282290148285348No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1150.12603706585710683No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1120.12274914239996493No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1120.12274914239996493No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT1120.12274914239996493No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1110.12165316791425097No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG1110.12165316791425097No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT1090.11946121894282301No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.11946121894282301No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCC1080.11836524445710903No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1070.11726926997139507No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1060.1161732954856811No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA1050.11507732099996712No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA1050.11507732099996712No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1040.11398134651425314No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1040.11398134651425314No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1030.11288537202853918No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1030.11288537202853918No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1010.11069342305711123No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1000.10959744857139725No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1000.10959744857139725No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1000.10959744857139725No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1000.10959744857139725No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC990.1085014740856833No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG980.10740549959996931No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC980.10740549959996931No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT950.1041175761428274No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC940.10302160165711342No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA940.10302160165711342No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT940.10302160165711342No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA940.10302160165711342No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA930.10192562717139944No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT920.10082965268568547No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTA920.10082965268568547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)