FastQCFastQC Report
Sun 21 May 2023
EGAF00007848510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38734
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8212.1195848608457686No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7161.848505189239428No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6741.7400733205968917No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6591.7013476532245573No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6111.5774255176330871No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6021.5541901172096866No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5901.523209583311819No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5781.4922290494139516No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5531.427686270460061No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5321.3734703361387928No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5221.3476532245572366No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4961.2805287344451903No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4901.2650384674962565No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4891.2624567563381008No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4711.2159859554912995No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4661.2030773997005215No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4521.1669334434863428No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4521.1669334434863428No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4431.143698043062942No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4361.1256260649558527No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4141.0688284194764288No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4121.0636649971601178No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4061.0481747302111841No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3921.0120307739970054No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3911.0094490628388495No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3901.0068673516806939No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3800.9810502400991378No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3770.973305106624671No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3690.9526514173594258No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3620.9345794392523363No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3510.9061806165126246No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3310.8545463933495121No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3270.8442195487168895No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3210.8287292817679558No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3200.8261475706098002No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3180.8209841482934889No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.8106573036608664No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2920.7538596581814426No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2830.7306242577580421No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.7022254350183301No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.6996437238601746No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2710.6996437238601746No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.6557546341715289No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.6531729230133733No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2480.6402643672225952No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2400.6196106779573501No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.5937935663757938No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2280.5886301440594827No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2260.5834667217431714No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2190.565394743636082No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.5499044766871483No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2030.5240873651055921No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.5163422316311251No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.5060153869985027No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1800.46470800846801263No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1790.462126297309857No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.44405431920276756No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1660.4285640522538338No Hit
TGCAGCACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1640.42340062993752264No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.41049207414674443No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.3950018071978107No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1470.37951154024887696No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1450.3743481179325657No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1440.3717664067744101No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.358857850983632No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.3536944286673207No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1360.35111271750916506No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1310.33820416171838696No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.3356224505602313No Hit
TGCAGCACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1290.3330407394020757No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1280.33045902824392004No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1280.33045902824392004No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1240.3201321836112976No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1190.30722362782051943No Hit
TGCAGCACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1170.3020602055042082No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1110.28656993855527446No Hit
TGCAGCACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.2839882273971188No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1070.2762430939226519No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1060.2736613827644963No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.2684979604481851No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1030.26591624929002944No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1030.26591624929002944No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1020.2633345381318738No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.26075282697371815No Hit
TGCAGCACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT950.24526256002478444No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT950.24526256002478444No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.24526256002478444No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC940.2426808488666288No Hit
TGCAGCACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT930.2400991377084732No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC920.23751742655031752No Hit
TGCAGCACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT920.23751742655031752No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG900.23235400423400632No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.22719058191769506No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA870.22460887075953945No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG870.22460887075953945No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA860.22202715960138378No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA830.2142820261269169No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT790.2039551814942944No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA790.2039551814942944No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.19879175917798317No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA760.19621004801982753No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG760.19621004801982753No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC760.19621004801982753No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA740.1910466257035163No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA730.18846491454536066No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC730.18846491454536066No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT730.18846491454536066No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA730.18846491454536066No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC720.18588320338720504No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT710.18330149222904943No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.17555635875458253No Hit
TGCAGCACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT680.17555635875458253No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA670.17297464759642692No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC670.17297464759642692No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA660.17039293643827128No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA650.16781122528011566No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG640.16522951412196002No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.16522951412196002No Hit
TGCAGCACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC630.1626478029638044No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.1626478029638044No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA630.1626478029638044No Hit
TGCAGCACCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.1600660918056488No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.15748438064749315No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT610.15748438064749315No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT610.15748438064749315No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA600.15490266948933754No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA600.15490266948933754No Hit
TGCAGCACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.14973924717302628No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.14457582485671502No Hit
TGCAGCACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT540.1394124025404038No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT540.1394124025404038No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT540.1394124025404038No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT530.13683069138224815No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT530.13683069138224815No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA520.13424898022409254No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT510.1316672690659369No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT510.1316672690659369No Hit
TGCAGCACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC500.12908555790778128No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.12650384674962567No Hit
TGCAGCACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT490.12650384674962567No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT490.12650384674962567No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.1213404244333144No Hit
TGCAGCACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.11875871327515876No Hit
TGCAGCACCCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT460.11875871327515876No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA460.11875871327515876No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA460.11875871327515876No Hit
TGCAGCACCCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT440.11359529095884753No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC430.11101357980069189No Hit
TGCAGCACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC420.10843186864253626No Hit
TGCAGCACCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.10843186864253626No Hit
TGCAGCACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC420.10843186864253626No Hit
TGCAGCACCCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT420.10843186864253626No Hit
TGCAGCACCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC410.10585015748438066No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.10585015748438066No Hit
TGCAGCACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.10326844632622502No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA400.10326844632622502No Hit
TGCAGCACCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT390.10068673516806939No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA390.10068673516806939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)