FastQCFastQC Report
Sun 21 May 2023
EGAF00007848514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48228
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA24415.061375134776478No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22914.750352492328108No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14973.1040059716347352No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14122.9277598075806583No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11032.2870531641370158No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9601.9905449116695697No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8531.768682093389732No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8031.6650078792402754No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7481.5509662436758729No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7421.5385253379779382No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7171.48668823090321No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6901.430704155262503No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6761.4016753753006552No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6681.385087501036742No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5751.1922534627187527No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5741.1901799784357634No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5531.1466368084929917No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5471.1341959027950568No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4991.0346686572115782No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4841.0035663929667413No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4770.9890520029858173No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4610.9558762544579913No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4590.951729285892013No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4510.9351414116280999No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4440.9206270216471759No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4430.9185535373641868No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4420.9164800530811976No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4360.9040391473832627No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4130.8563490088745127No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3940.8169528074977191No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3820.7920709961018495No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3800.7879240275358712No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3670.7609687318570125No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3560.738160404744132No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3500.7257194990461973No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3430.7112051090652733No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3170.6572945177075558No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.6427801277266318No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3090.6407066434436427No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3080.6386331591606536No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2950.6116778634817948No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2870.5950899892178817No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2800.5805755992369578No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2750.5702081778220122No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2750.5702081778220122No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2650.5494733349921207No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2620.5432528821431534No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2590.5370324292941859No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2560.5308119764452186No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2500.5183710707472837No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2440.505930165049349No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2370.491415775068425No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2340.4851953222194576No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2320.4810483536534793No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.4437256365596749No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2130.44165215227668575No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2130.44165215227668575No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2020.41884382516380525No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1980.4105498880318487No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1980.4105498880318487No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1930.40018246661690304No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1920.3981089823339139No Hit
TACCTCGTACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1920.3981089823339139No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1830.3794476237870117No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1810.37530065522103345No Hit
TACCTCGTACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.35871278095712034No Hit
TACCTCGTACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.3421249066932073No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1640.34005142241021813No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1590.32968400099527245No Hit
TACCTCGTACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.3276105167122833No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1540.3193165795803268No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1490.3089491581653811No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1460.3027287053164137No Hit
TACCTCGTACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.2716264410715767No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1300.26955295678858754No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1280.2654059882226093No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.261259019656631No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1240.2571120510906527No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1190.24674462967570707No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1170.2425976611097288No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.24052417682673966No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1060.21978933399684827No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1040.21564236543087004No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1020.21149539686489177No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.20942191258190263No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1000.20734842829891348No Hit
TACCTCGTACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT990.20527494401592436No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC970.20112797544994612No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT940.19490752260097868No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA900.18661358546902215No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA870.18039313262005474No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA870.18039313262005474No Hit
TACCTCGTACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT870.18039313262005474No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.17831964833706562No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA850.17624616405407648No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT840.17417267977108733No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC840.17417267977108733No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT840.17417267977108733No Hit
TACCTCGTACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.1720991954880982No Hit
TACCTCGTACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.1720991954880982No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA820.17002571120510906No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.16795222692211992No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT800.1658787426391308No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA760.15758480550717427No Hit
TACCTCGTACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT760.15758480550717427No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA750.15551132122418512No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC740.15343783694119598No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.15136435265820686No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.1472173840922286No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA710.1472173840922286No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA700.14514389980923945No Hit
TACCTCGTACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT700.14514389980923945No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.1430704155262503No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT690.1430704155262503No Hit
TACCTCGTACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT680.14099693124326118No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.13892344696027203No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT670.13892344696027203No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA670.13892344696027203No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC660.1368499626772829No Hit
TACCTCGTACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.13477647839429377No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA640.13270299411130465No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC620.12855602554532636No Hit
TACCTCGTACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.12855602554532636No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG620.12855602554532636No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.12855602554532636No Hit
TACCTCGTACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.12648254126233724No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA610.12648254126233724No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT610.12648254126233724No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.12648254126233724No Hit
TACCTCGTACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC600.1244090569793481No Hit
TACCTCGTACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.1244090569793481No Hit
TACCTCGTACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT590.12233557269635896No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.12026208841336983No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.12026208841336983No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.12026208841336983No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA560.11611511984739155No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA560.11611511984739155No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA560.11611511984739155No Hit
TACCTCGTACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT560.11611511984739155No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA550.11404163556440242No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA540.1119681512814133No Hit
TACCTCGTACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC540.1119681512814133No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA530.10989466699842414No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT520.10782118271543502No Hit
TACCTCGTACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC520.10782118271543502No Hit
TACCTCGTACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT520.10782118271543502No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA510.10574769843244589No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA510.10574769843244589No Hit
TACCTCGTACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.10367421414945674No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA500.10367421414945674No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA500.10367421414945674No Hit
TACCTCGTACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT490.1016007298664676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)