FastQCFastQC Report
Fri 2 Jun 2023
EGAF00007848518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152568683
Sequences flagged as poor quality0
Sequence length75
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA51764713.3928791271010708TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT4259040.2791555852913799TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2439870.15991945083513634TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2410390.15798720632595353TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA2166210.14198261120206432TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)