FastQCFastQC Report
Fri 13 Jan 2023
EGAF00007848526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77347432
Sequences flagged as poor quality0
Sequence length75
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA28656903.7049581684883863TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT2538550.3282009414352631TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1788010.23116604569366955TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1613240.20857059611235704TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA1058230.13681514339092732TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC808980.10459041484402481TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT781470.10103373567722326TruSeq Adapter, Index 12 (100% over 55bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)