FastQCFastQC Report
Thu 16 May 2024
EGAF00007848526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77347432
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC39783685.143503665383486TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC964570.12470614409021362TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGATCGGAAGAGCACACGTCTGAACTCCAGT832690.10765580426768402Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC778160.10060579645359137TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)