FastQCFastQC Report
Sun 12 May 2024
EGAF00007848534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56967550
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC669831111.758116682216455TruSeq Adapter, Index 12 (100% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1470470.25812414260399125TruSeq Adapter, Index 12 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1205500.21161169823873416TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGATCGGAAGAGCACACGTCTGAACTCCAGT1150630.20197989908289893Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCT700490.12296298506781493TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)