FastQCFastQC Report
Fri 3 May 2024
EGAF00007848535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007848535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56967550
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG649894011.408143759034749Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGAAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1218320.21386210219677695Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1181670.20742861506243465Illumina Single End PCR Primer 1 (100% over 50bp)
GATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1104990.19396832056144245Illumina Single End PCR Primer 1 (98% over 50bp)
GACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1088710.19111055328867046Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)