FastQCFastQC Report
Mon 22 May 2023
EGAF00007850096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007850096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3857615
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG453191.174793233643067TruSeq Adapter, Index 19 (97% over 37bp)
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC262030.679253891329228No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC250280.6487946568021952No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC224700.5824842551680248No Hit
CGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC130360.3379290053569369No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA126370.3275858269941402No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG123810.32094960228016534No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC121720.3155317469472718No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT121000.3136653087464664No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA115250.298759725892812No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC101430.26293448153846355No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT93280.24180743801545773No Hit
CAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACT86490.22420588887175105No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC84020.21780296893287693No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG82440.21370717399222058No Hit
TGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG79960.20727833130055745No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA71810.1861512877775517No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA62960.16320965155931838No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA59810.15504398443079467No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT58240.1509741122429273No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG58180.15081857572619353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57300.14853737348076468No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT57000.14775969089709573No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT53390.13840157714027967No Hit
GAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACTC52950.13726097601756526No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG52080.13500569652492536No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG51010.13223196197650622No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT50300.13039144652848977No Hit
GGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG48700.1262438060822555No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT48480.12567350552089826No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC47520.1231849212531577No Hit
AGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTT46710.12108517827725163No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG45180.1171189971005401No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC41630.10791641986045782No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC40390.10470199851462626No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC40020.1037428566614346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)