FastQCFastQC Report
Wed 8 May 2024
EGAF00007850108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007850108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3651266
Total Bases368.7 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG372381.0198654384534023TruSeq Adapter, Index 19 (97% over 37bp)
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC278700.7632968948304506No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC265390.7268437851419206No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC230570.6314796018695981No Hit
CGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC141420.3873177139107367No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG133670.36609219925362874No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC131700.3606968103665961No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA131650.3605598715623567No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT113800.3116727184488887No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA113260.3101937793631031No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT99430.2723165061104833No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC96350.2638810757693359No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG91750.25128270577931056No Hit
CAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACT88680.242874663199011No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC88490.24235429574290124No Hit
TGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG82440.22578470042993307No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA75160.2058464105326755No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA66140.18114265024788664No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA63110.1728441587109786No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT57520.15753440039701297No Hit
GAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACTC54930.15044097033741174No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG53860.14751047992668842No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT53180.14564811218903254No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG52270.14315582595187532No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT50360.13792476362993No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT50000.13693880423940627No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC48840.13376182398105205No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT48140.13184468072170036No Hit
GGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG47490.1300644762665881No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC43920.12028704564389447No Hit
AGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTT43250.11845206566708642No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC42780.11716484090723601No Hit
GGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG42470.1163158203209517No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG39080.10703136939351995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)